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Hi Bradford,</div>
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<span style="color: rgb(0, 0, 0); font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;">In our lab, we have been using poly-L-Lysine as a positive substrate and it has yielded good results consistently with a clear background. </span><br>
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<span style="color: rgb(0, 0, 0); font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;">Chen</span></div>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Ross, Bradford <bradford.ross@botany.ubc.ca><br>
<b>Sent:</b> Wednesday, July 3, 2019 14:06<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] How to make grids positively charged during or after glow discharge.</font>
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<p>Hello Everyone,</p>
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<p>I'm currently trying to image some lipid nanoparticles which have a positive charge on their outer surface. Of course, since normal glow discharge treatment typically yields a negative charge on the grids, the particles are all sticking to the lacey carbon
support instead of remaining suspended in the vitreous ice layer.</p>
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<p>I have heard that Magnesium Acetate can be used to switch the charge from negative to positive after glow discharging, but can't seem to find any protocols for exactly how to treat the grids with it.
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<p>Does anyone have a protocol for the use of Magnesium Acetate, or any other suggestions on how to flip the surface charge of the grids to positive? Or other creative solutions I'm not thinking of?<br>
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<p>Cheers,<br>
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<div style="font-family:Tahoma; font-size:13px"><font size="2">Bradford Ross<br>
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Electron Microscopy Technician<br>
BioImaging Facility<br>
University of British Columbia<br>
Cunningham Building Rm. 64<br>
2146 East Mall <br>
Vancouver, B.C. <br>
V6T 1Z4<br>
<br>
phone 604-822-6996<br>
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