[3dem] bad background in K2 images

[Craig Yoshioka]

Agreed, flip or 180º rotation.

On Jan 28, 2019, at 8:50 PM, Peter Shen <peter.shen at biochem.utah.edu<mailto:peter.shen at biochem.utah.edu>> wrote:

Hi Garry-- Looks like you just need to apply a vertical flip to your gain file.

-Peter
________________________________
From: 3dem [3dem-bounces at ncmir.ucsd.edu<mailto:3dem-bounces at ncmir.ucsd.edu>] on behalf of Garry P Morgan [Garry.Morgan at Colorado.EDU<mailto:Garry.Morgan at Colorado.EDU>]
Sent: Monday, January 28, 2019 6:36 PM
To: 3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>
Subject: [3dem] bad background in K2 images

Hello,
we have been experiencing some strange patterns in our motion-corrected K2 data (see attached images)…these were collected using super-res mode and processed in cryo-sparc, so they are quite heavily filtered, which really accentuates the wavy pattern, which is not even noticeable when observing an aligned/summed images from SerialEM or GMS. has anyone seen this or something similar with their K2 acquired data?  and are there any ideas on how to fix this?
thanks,
Garry


<image001.png><image002.png>


________________________________
Garry Morgan
Director  —  Boulder EM Services —  Department of MCD Biology
Campus Box 347  —  University of Colorado  —  Boulder, CO 80309
phone:  303-492-8402

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