[3dem] bad background in K2 images

[Peter Shen]

Hi Garry-- Looks like you just need to apply a vertical flip to your gain file.

-Peter
________________________________
From: 3dem [3dem-bounces at ncmir.ucsd.edu] on behalf of Garry P Morgan [Garry.Morgan at Colorado.EDU]
Sent: Monday, January 28, 2019 6:36 PM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] bad background in K2 images

Hello,
we have been experiencing some strange patterns in our motion-corrected K2 data (see attached images)…these were collected using super-res mode and processed in cryo-sparc, so they are quite heavily filtered, which really accentuates the wavy pattern, which is not even noticeable when observing an aligned/summed images from SerialEM or GMS. has anyone seen this or something similar with their K2 acquired data?  and are there any ideas on how to fix this?
thanks,
Garry


[cid:7B1F95F5-163F-4053-8CCE-CED85488ECB1 at Home][cid:5FF3CCAF-F39C-4923-BB5F-A84E0CFBD069 at Home]


________________________________
Garry Morgan
Director  —  Boulder EM Services —  Department of MCD Biology
Campus Box 347  —  University of Colorado  —  Boulder, CO 80309
phone:  303-492-8402

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