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<div style="direction: ltr;font-family: Tahoma;color: #000000;font-size: 10pt;">Hi Garry-- Looks like you just need to apply a vertical flip to your gain file.
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<div>-Peter<br>
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<div id="divRpF482089" style="direction: ltr;"><font face="Tahoma" size="2" color="#000000"><b>From:</b> 3dem [3dem-bounces@ncmir.ucsd.edu] on behalf of Garry P Morgan [Garry.Morgan@Colorado.EDU]<br>
<b>Sent:</b> Monday, January 28, 2019 6:36 PM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] bad background in K2 images<br>
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<div>Hello,
<div class="">we have been experiencing some strange patterns in our motion-corrected K2 data (see attached images)…these were collected using super-res mode and processed in cryo-sparc, so they are quite heavily filtered, which really accentuates the wavy
pattern, which is not even noticeable when observing an aligned/summed images from SerialEM or GMS. has anyone seen this or something similar with their K2 acquired data? and are there any ideas on how to fix this?</div>
<div class="">thanks,</div>
<div class="">Garry</div>
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<div class=""><img id="7A2C75FB-F9EF-4CDB-8523-92FB4CDB2EFE" src="cid:7B1F95F5-163F-4053-8CCE-CED85488ECB1@Home" class=""><img id="0D106929-A98A-46C7-80C9-AFE9F328EE52" src="cid:5FF3CCAF-F39C-4923-BB5F-A84E0CFBD069@Home" class=""></div>
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<div class="">________________________________<br class="">
Garry Morgan<br class="">
Director — Boulder EM Services — Department of MCD Biology<br class="">
Campus Box 347 — University of Colorado — Boulder, CO 80309<br class="">
phone: 303-492-8402 </div>
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