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Agreed, flip or 180º rotation. <br class="">
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<div class="">On Jan 28, 2019, at 8:50 PM, Peter Shen <<a href="mailto:peter.shen@biochem.utah.edu" class="">peter.shen@biochem.utah.edu</a>> wrote:</div>
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Hi Garry-- Looks like you just need to apply a vertical flip to your gain file.
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<div class="">-Peter<br class="">
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<div id="divRpF482089" style="direction: ltr;" class=""><font face="Tahoma" size="2" class=""><b class="">From:</b><span class="Apple-converted-space"> </span>3dem [<a href="mailto:3dem-bounces@ncmir.ucsd.edu" class="">3dem-bounces@ncmir.ucsd.edu</a>] on behalf
of Garry P Morgan [<a href="mailto:Garry.Morgan@Colorado.EDU" class="">Garry.Morgan@Colorado.EDU</a>]<br class="">
<b class="">Sent:</b><span class="Apple-converted-space"> </span>Monday, January 28, 2019 6:36 PM<br class="">
<b class="">To:</b><span class="Apple-converted-space"> </span><a href="mailto:3dem@ncmir.ucsd.edu" class="">3dem@ncmir.ucsd.edu</a><br class="">
<b class="">Subject:</b><span class="Apple-converted-space"> </span>[3dem] bad background in K2 images<br class="">
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<div class="">Hello,
<div class="">we have been experiencing some strange patterns in our motion-corrected K2 data (see attached images)…these were collected using super-res mode and processed in cryo-sparc, so they are quite heavily filtered, which really accentuates the wavy
pattern, which is not even noticeable when observing an aligned/summed images from SerialEM or GMS. has anyone seen this or something similar with their K2 acquired data? and are there any ideas on how to fix this?</div>
<div class="">thanks,</div>
<div class="">Garry</div>
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<div class=""><span id="cid:7B1F95F5-163F-4053-8CCE-CED85488ECB1@Home"><image001.png></span><span id="cid:5FF3CCAF-F39C-4923-BB5F-A84E0CFBD069@Home"><image002.png></span></div>
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<div class="">________________________________<br class="">
Garry Morgan<br class="">
Director — Boulder EM Services — Department of MCD Biology<br class="">
Campus Box 347 — University of Colorado — Boulder, CO 80309<br class="">
phone: 303-492-8402<span class="Apple-converted-space"> </span></div>
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<span style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; float: none; display: inline !important;" class="">_______________________________________________</span><br style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;" class="">
<span style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; float: none; display: inline !important;" class="">3dem
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