[3dem] Recommendation of CTF estimation program

[Carlos Oscar S. Sorzano]

Remind that the best way is to use Xmipp through Scipion.

Kind regards, Carlos Oscar

El 15/02/2019 a las 0:08, Guobin Hu escribió:
> Dear Carlos,
>
> Thank you very much for your feedback and suggestion. Answering your 
> question. Yes. I see Thon rings in FFT. I would check out Xmipp.
>
> Best,
> Guobin
>
> Sent from my iPhone, please excuse typos
>
> On Feb 14, 2019, at 5:07 PM, Carlos Oscar S. Sorzano <coss at cnb.csic.es 
> <mailto:coss at cnb.csic.es>> wrote:
>
>> Dear Guobin,
>>
>> stated like this, it is to difficult to give an "absolute" 
>> recommendation. A first question would be if you see the Thon rings 
>> by eye? If not, I would say that most programs will not be able to 
>> find the defocus. If you can see them, then it is a matter of trying. 
>> All I can offer in this regard is to try Xmipp on this, if you make 
>> one or two of these micrographs available, you may send me a personal 
>> message with the link and we will check them.
>>
>> Kind regards, Carlos Oscar
>>
>> El 14/02/2019 a las 21:40, Guobin Hu escribió:
>>> Dear Cryo-EM folks,
>>>
>>> I am dealing with some micrographs of very low defocus values e.g. a 
>>> few tens of nanometers. I found it sometimes is difficult for the 
>>> programs I am using to find the right defocus value even I set the 
>>> corresponding defocus search range. I see a lot of CTF estimation 
>>> programs on the wikipage. I do not want to download all of them and 
>>> try one by one. I would greatly appreciate it if somebody can 
>>> recommend one that works well with lowly defocused micrographs. The 
>>> best is the program can also display the Thon rings of CTF of very 
>>> small defocus values.
>>>
>>> Best,
>>> Guobin Hu
>>>
>>> _______________________________________________
>>> 3dem mailing list
>>> 3dem at ncmir.ucsd.edu
>>> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>> -- 
>> ------------------------------------------------------------------------
>> Carlos Oscar Sánchez Sorzano                  e-mail:coss at cnb.csic.es
>> Biocomputing unithttp://i2pc.es/coss
>> National Center of Biotechnology (CSIC)
>> c/Darwin, 3
>> Campus Universidad Autónoma (Cantoblanco)     Tlf: 34-91-585 4510
>> 28049 MADRID (SPAIN)                          Fax: 34-91-585 4506
>> ------------------------------------------------------------------------
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-- 
------------------------------------------------------------------------
Carlos Oscar Sánchez Sorzano                  e-mail:   coss at cnb.csic.es
Biocomputing unit                             http://i2pc.es/coss
National Center of Biotechnology (CSIC)
c/Darwin, 3
Campus Universidad Autónoma (Cantoblanco)     Tlf: 34-91-585 4510
28049 MADRID (SPAIN)                          Fax: 34-91-585 4506
------------------------------------------------------------------------

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