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<p>Remind that the best way is to use Xmipp through Scipion.</p>
<p>Kind regards, Carlos Oscar<br>
</p>
<div class="moz-cite-prefix">El 15/02/2019 a las 0:08, Guobin Hu
escribió:<br>
</div>
<blockquote type="cite"
cite="mid:D26533F2-1F87-4393-9682-30BA6B48B75A@yahoo.com">
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Dear Carlos,
<div><br>
</div>
<div>Thank you very much for your feedback and suggestion.
Answering your question. Yes. I see Thon rings in FFT. I would
check out Xmipp. </div>
<div><br>
</div>
<div>Best,</div>
<div>Guobin<br>
<br>
<div id="AppleMailSignature" dir="ltr">Sent from my iPhone,
please excuse typos</div>
<div dir="ltr"><br>
On Feb 14, 2019, at 5:07 PM, Carlos Oscar S. Sorzano <<a
href="mailto:coss@cnb.csic.es" moz-do-not-send="true">coss@cnb.csic.es</a>>
wrote:<br>
<br>
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<p>Dear Guobin,</p>
<p>stated like this, it is to difficult to give an
"absolute" recommendation. A first question would be if
you see the Thon rings by eye? If not, I would say that
most programs will not be able to find the defocus. If you
can see them, then it is a matter of trying. All I can
offer in this regard is to try Xmipp on this, if you make
one or two of these micrographs available, you may send me
a personal message with the link and we will check them.</p>
<p>Kind regards, Carlos Oscar<br>
</p>
<div class="moz-cite-prefix">El 14/02/2019 a las 21:40,
Guobin Hu escribió:<br>
</div>
<blockquote type="cite"
cite="mid:774657896.1137089.1550176824857@mail.yahoo.com">
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charset=UTF-8">
<div>Dear Cryo-EM folks,</div>
<div><br>
</div>
<div>I am dealing with some micrographs of very low
defocus values e.g. a few tens of nanometers. I found it
sometimes is difficult for the programs I am using to
find the right defocus value even I set the
corresponding defocus search range. I see a lot of CTF
estimation programs on the wikipage. I do not want to
download all of them and try one by one. I would greatly
appreciate it if somebody can recommend one that works
well with lowly defocused micrographs. The best is the
program can also display the Thon rings of CTF of very
small defocus values. </div>
<div><br>
</div>
<div>Best,</div>
<div>Guobin Hu </div>
<br>
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<pre class="moz-signature" cols="72">--
------------------------------------------------------------------------
Carlos Oscar Sánchez Sorzano e-mail: <a class="moz-txt-link-abbreviated" href="mailto:coss@cnb.csic.es" moz-do-not-send="true">coss@cnb.csic.es</a>
Biocomputing unit <a class="moz-txt-link-freetext" href="http://i2pc.es/coss" moz-do-not-send="true">http://i2pc.es/coss</a>
National Center of Biotechnology (CSIC)
c/Darwin, 3
Campus Universidad Autónoma (Cantoblanco) Tlf: 34-91-585 4510
28049 MADRID (SPAIN) Fax: 34-91-585 4506
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<pre class="moz-signature" cols="72">--
------------------------------------------------------------------------
Carlos Oscar Sánchez Sorzano e-mail: <a class="moz-txt-link-abbreviated" href="mailto:coss@cnb.csic.es">coss@cnb.csic.es</a>
Biocomputing unit <a class="moz-txt-link-freetext" href="http://i2pc.es/coss">http://i2pc.es/coss</a>
National Center of Biotechnology (CSIC)
c/Darwin, 3
Campus Universidad Autónoma (Cantoblanco) Tlf: 34-91-585 4510
28049 MADRID (SPAIN) Fax: 34-91-585 4506
------------------------------------------------------------------------
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