[3dem] Using SerialEM and Leginon on same scope

Rice, William William.Rice at nyulangone.org
Fri Aug 30 10:32:07 PDT 2019


Hi Bob,


I can also say that they coexist well at NYU, where we have a Krios and an Arctica. As Matthias said, I don't run them at the same time. We also have EPU on the Krios but it is very seldomly used (only for the Falcon 3 -- no advanced scripting installed yet)


Bill


William J. Rice, PhD

Director, Cryo-Electron Microscopy Laboratory

NYU Langone Health

Smilow, SR C-10

522 First Ave., New York, NY 10016

________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Grassucci, Robert <rg2502 at cumc.columbia.edu>
Sent: Friday, August 30, 2019 9:44:59 AM
To: 3DEM List (3dem at ncmir.ucsd.edu)
Subject: [3dem] Using SerialEM and Leginon on same scope

Hi All,
With the commissioning of our 4 new scopes we are debating if we should put SerialEM and Leginon on the same microscopes.  I have heard from some people that there may be some problems that arise during the transition.  We have mostly been using Leginon so have a great deal of experience with it and use SerialEM for our limited tomography users.  I cannot see any reason they should conflict but want to ask the 3DEM community if they have any specific experience where this has been a problem.  Thank you and I look forward to your advice.
Regards,
Bob


Robert Grassucci
Co Director CryoEM Core Facility
Columbia University Medical Center
Biochemistry and Molecular Biophysics Dept.
Black Building 2-221
650 W 168 Street
New York, NY 10032
Phone: 212-305-9520

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