[3dem] Using SerialEM and Leginon on same scope

[Ho, Chi-Min]

Hi Bob,

We (in the Zhou Lab) run both SerialEM and Leginon on our Krios at UCLA quite regularly and have no problems switching back and forth. Best of luck!

Best wishes,
~Mimi

Chi-Min Ho, Ph.D.
Assistant Professor of Microbiology & Immunology
Columbia University, Vagelos College of Physicians and Surgeons

Columbia University Irving Medical Center
701 West 168th Street
Hammer Health Sciences Building
New York, NY 10032
www.cmholab.org<http://www.cmholab.org/>

________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Grassucci, Robert <rg2502 at cumc.columbia.edu>
Sent: Friday, August 30, 2019 9:44 AM
To: 3DEM List (3dem at ncmir.ucsd.edu) <3dem at ncmir.ucsd.edu>
Subject: [3dem] Using SerialEM and Leginon on same scope


Hi All,

With the commissioning of our 4 new scopes we are debating if we should put SerialEM and Leginon on the same microscopes.  I have heard from some people that there may be some problems that arise during the transition.  We have mostly been using Leginon so have a great deal of experience with it and use SerialEM for our limited tomography users.  I cannot see any reason they should conflict but want to ask the 3DEM community if they have any specific experience where this has been a problem.  Thank you and I look forward to your advice.

Regards,

Bob





Robert Grassucci

Co Director CryoEM Core Facility

Columbia University Medical Center

Biochemistry and Molecular Biophysics Dept.

Black Building 2-221

650 W 168 Street

New York, NY 10032

Phone: 212-305-9520


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