[3dem] Using SerialEM and Leginon on same scope

[Matthias Wolf]

Hi Bob,

no conflict. We have SerialEM, Leginon, EPU and FEI Tomo4. They coexist fine.
Only requirement for Falcon is that you will need advanced scripting for access to the frames on the Falcon storage server.
For serialEM, you must run the serialem server on the microscope PC, and for leginon the leginon client. If you have a K2, you also need to run a second leginon client on the camera PC.
Of course running all these clients all at the same time may confuse the system, but who would do that.

I would standardize on no more than two of them, because you will need calibrations for each scope and each camera on each system - it quickly gets out of hand. In this respect, EPU and Tomo win, because TFS will do the installation and calibration for you. I think SerialEM is best for tomo, because David is always at the forefront when it comes to implementing new insights - such as the dose symmetric scheme. It's got great tracking and it seamlessly integrates with IMOD.

   Matthias
________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Grassucci, Robert <rg2502 at cumc.columbia.edu>
Sent: Friday, August 30, 2019 10:44 PM
To: 3DEM List (3dem at ncmir.ucsd.edu) <3dem at ncmir.ucsd.edu>
Subject: [3dem] Using SerialEM and Leginon on same scope


Hi All,

With the commissioning of our 4 new scopes we are debating if we should put SerialEM and Leginon on the same microscopes.  I have heard from some people that there may be some problems that arise during the transition.  We have mostly been using Leginon so have a great deal of experience with it and use SerialEM for our limited tomography users.  I cannot see any reason they should conflict but want to ask the 3DEM community if they have any specific experience where this has been a problem.  Thank you and I look forward to your advice.

Regards,

Bob





Robert Grassucci

Co Director CryoEM Core Facility

Columbia University Medical Center

Biochemistry and Molecular Biophysics Dept.

Black Building 2-221

650 W 168 Street

New York, NY 10032

Phone: 212-305-9520


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