[3dem] 3dem Digest, Vol 133, Issue 16

Jason Kaelber jason.kaelber at rutgers.edu
Fri Sep 7 11:45:48 PDT 2018 

Stan,

Pelco recently introduced a rubber protector for the jar, part no. 91053. Vlad Speransky mentioned this on the listserv a while ago; since adding it my users haven't chipped any jars (vs. !@#$ many jars before).

Sometimes the easiGlow protocol terminates without venting all the way. Then the user should open the gas inlet via the manual control in the options menu.


Kristin,

We have an "all-in-one" sort of unit in addition to the easiGlow. The thing is that adding the glow discharge feature to the fancy unit cost us about the same as buying a stand-alone glow discharger!

Also, if you anticipate doing controlled-atmosphere discharge, such as pentylamine, it is more cost-effective (and space-effective) to get a double-chamber unit upfront rather than having to get a second unit later. Corduan and Pelco both offer a double-chamber and I bet others do as well.


Cheers,

Jason Kaelber

Rutgers

________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of 3dem-request at ncmir.ucsd.edu <3dem-request at ncmir.ucsd.edu>
Sent: Friday, September 7, 2018 2:21:32 PM
To: 3dem at ncmir.ucsd.edu
Subject: 3dem Digest, Vol 133, Issue 16

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Today's Topics:

   1. questions about carbon coaters /glow discharge (Parent, Kristin)
   2. Re: questions about carbon coaters /glow discharge
      (Stanislav Vitha)


----------------------------------------------------------------------

Message: 1
Date: Fri, 7 Sep 2018 17:37:06 +0000
From: "Parent, Kristin" <kparent at msu.edu>
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: [3dem] questions about carbon coaters /glow discharge
Message-ID: <498F1D10-1C94-4D86-A79E-39341A983BCC at msu.edu>
Content-Type: text/plain; charset="utf-8"

Hello all,

I was hoping some of you might share good/bad experiences with a carbon coater & glow discharge device? We are planning to purchase one to support our growing facility and I would love to hear this groups? experience.

We have on hand a plasma cleaner that works really well, so what I am hoping for is ideally something to supplement our existing equipment (for those that prefer glow discharge) and also is capable of carbon coating.

Thank you in advance,
Kristin

--
Kristin N. Parent, Ph.D.
J. K. Billman, Jr., M.D. Endowed Research Professor
and Assistant Professor of Biochemistry & Molecular Biology
Michigan State University
603 Wilson Road
East Lansing, MI 48824
Office phone: (517) 432-8434
https://na01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fkparentlab.natsci.msu.edu%2F&data=02%7C01%7Cjason.kaelber%40rutgers.edu%7Caa30920cf3944f59c03e08d614eed5f7%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C636719413399150766&sdata=jv9pZzpVtqLeeDzLHHiyM5lSZ%2F%2Byi2AiGXeM2dSMoK8%3D&reserved=0
Twitter: @Phage4Lyfe


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Message: 2
Date: Fri, 7 Sep 2018 18:21:22 +0000
From: Stanislav Vitha <stanvitha at tamu.edu>
To: "'3dem at ncmir.ucsd.edu'" <3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] questions about carbon coaters /glow discharge
Message-ID: <63557b559b624891a06873ae6c3f9aab at tamu.edu>
Content-Type: text/plain; charset="utf-8"

We have the Pelco EasiGlow  glow discharge apparatus, separate from a Cressington carbon coater (with which I do not have experience).
The EasiGlow has worked without problems for many years  (9  years or more), and users love the easy operation, with a single button to push.

The only issue is that some users are a bit impatient and try to yank off the bell jar before the vacuum is fully released and doing so chip the edge of the bell jar (which then does not seal).? Filling the chipped area with JB Weld and sanding it smooth fixed it many times, but eventually we had to buy a new jar.

Go Spartans!

Regards,

Stan

Dr. Stanislav Vitha
Research Scientist
Microscopy and Imaging Center
Texas A&M University
301 Old Main Dr
College Station, TX 77843-2257

Office: ILSB Room 1131
<https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fmicroscopy.tamu.edu%2F&data=02%7C01%7Cjason.kaelber%40rutgers.edu%7Caa30920cf3944f59c03e08d614eed5f7%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C636719413399150766&sdata=jFTp7GFvjt2Csuc9hRHebP5M%2FnzFPPUsjTzOxbWwH5o%3D&reserved=0>Tel. 979-845-1607
https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Fmicroscopy.tamu.edu&data=02%7C01%7Cjason.kaelber%40rutgers.edu%7Caa30920cf3944f59c03e08d614eed5f7%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C636719413399150766&sdata=3aTmoznJXV6M%2FA1JExtjkLU%2FSMrRU1Psfyk%2FLluQD8I%3D&reserved=0



From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of Parent, Kristin
Sent: Friday, September 07, 2018 12:37 PM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] questions about carbon coaters /glow discharge

Hello all,

I was hoping some of you might share good/bad experiences with a carbon coater & glow discharge device? We are planning to purchase one to support our growing facility and I would love to hear this groups? experience.

We have on hand a plasma cleaner that works really well, so what I am hoping for is ideally something to supplement our existing equipment (for those that prefer glow discharge) and also is capable of carbon coating.

Thank you in advance,
Kristin

--
Kristin N. Parent, Ph.D.
J. K. Billman, Jr., M.D. Endowed Research Professor
and Assistant Professor of Biochemistry & Molecular Biology
Michigan State University
603 Wilson Road
East Lansing, MI 48824
Office phone: (517) 432-8434
https://na01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fkparentlab.natsci.msu.edu%2F&data=02%7C01%7Cjason.kaelber%40rutgers.edu%7Caa30920cf3944f59c03e08d614eed5f7%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C636719413399150766&sdata=jv9pZzpVtqLeeDzLHHiyM5lSZ%2F%2Byi2AiGXeM2dSMoK8%3D&reserved=0<https://na01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttps-3A__kparentlab.natsci.msu.edu_%26d%3DDwMGaQ%26c%3DODFT-G5SujMiGrKuoJJjVg%26r%3DtE-7MR_zhznDCk27UIOmF3KCvigbMQUT9WjrucIisxY%26m%3DAeieE4STXjyYpq7quSIuHQT-42yy1OnpTXbLyBrQbUs%26s%3DmsFGECctI8t4RODwkc4-PTKfaS1Yiybkn57L725GuwU%26e&data=02%7C01%7Cjason.kaelber%40rutgers.edu%7Caa30920cf3944f59c03e08d614eed5f7%7Cb92d2b234d35447093ff69aca6632ffe%7C1%7C0%7C636719413399150766&sdata=d6DOiT6c21jPE54pUAuwfh0edjCKA8bKxuNeQbpWXUE%3D&reserved=0=>
Twitter: @Phage4Lyfe


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