[3dem] Leaking loading stations

[jamonshi]

After our two loading stations broke and started leaking, I had local 
machine shop replaced them with metal inlet. It burns out more LN2 at 
beginning, but frost not so big issue since our room humidity are 
controlled within 30-40%.

Regards,
Jian
On 07/09/2018 00:19, Claudia Lopez wrote:
> Hi Michael,
> Yes, this happened to us on several transfer units. We received a prototype from TFS in which the insert is made out of metal (see attachment). This one of course doesn’t crack, but it burns LN2 much faster and if you don’t have a well-controlled environment there is plenty of frost build up. I am not sure they are still working on this other type of transfer station or not.
>
> Also, we stopped baking our units (37 C) and this has prevented, so far, the plastic transfer units we still own to crack. The other thing you want to make sure is not to overfill the transfer station.
> Regards,
>
> Claudia
>
> Claudia S. López, PhD
> Research Assistant Professor
> Department of Biomedical Engineering
> Director Multiscale Microscopy Core
> Co-Manager Pacific Northwest  Center for Cryo-EM (PNCC).
>
> Phone: 503-418-0186
> Oregon Health & Science University
> LVMS Mail Code: CL-P2M
> 2730 SW Moody Ave
> Portland OR 97201
>
>
>
>
>
>
> -----Original Message-----
> From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of Michael Braunfeld
> Sent: Thursday, September 06, 2018 8:59 AM
> To: 3dem at ncmir.ucsd.edu
> Subject: [3dem] Leaking loading stations
>
> Hi All,
>
> Do other facilities have any issues with the loading stations for the Krios/Arctica? We just experienced our second failure in the last month.
> The LN2 is leaking out at a pretty rapid rate from cracks at the base of the stations. These are not being abused in any way (as far as I know), and the worst part is the price quote from Thermo.
>
>
> Thanks,
>
> Michael Braunfeld
>
> HHMI/UCSF
>
>
>
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