[3dem] Leaking loading stations

[Claudia Lopez]

Hi Michael,
Yes, this happened to us on several transfer units. We received a prototype from TFS in which the insert is made out of metal (see attachment). This one of course doesn’t crack, but it burns LN2 much faster and if you don’t have a well-controlled environment there is plenty of frost build up. I am not sure they are still working on this other type of transfer station or not. 

Also, we stopped baking our units (37 C) and this has prevented, so far, the plastic transfer units we still own to crack. The other thing you want to make sure is not to overfill the transfer station.
Regards,

Claudia

Claudia S. López, PhD
Research Assistant Professor
Department of Biomedical Engineering
Director Multiscale Microscopy Core
Co-Manager Pacific Northwest  Center for Cryo-EM (PNCC).

Phone: 503-418-0186
Oregon Health & Science University
LVMS Mail Code: CL-P2M
2730 SW Moody Ave
Portland OR 97201






-----Original Message-----
From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of Michael Braunfeld
Sent: Thursday, September 06, 2018 8:59 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Leaking loading stations

Hi All,

Do other facilities have any issues with the loading stations for the Krios/Arctica? We just experienced our second failure in the last month. 
The LN2 is leaking out at a pretty rapid rate from cracks at the base of the stations. These are not being abused in any way (as far as I know), and the worst part is the price quote from Thermo.


Thanks,

Michael Braunfeld

HHMI/UCSF

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