[3dem] Add random noise to reconstruction

[Steinar Halldorsson]

Hi all


I have something which looks like an artefact in my FSC curves from tomography data calculated in Relion. Basically the curves hover around 0.1 instead of reaching 0... And this is not coming from my mask btw. There is quite a lot of information (protein) outside of the mask, you can see clear but slightly smeary density from neighbours in the final maps, and I wonder if this is causing these correlations? One way I have thought to test this is to take my two half maps, somehow add random noise to parts outside of my region of interest and then recalculate my FSC.

Firstly, does this sound like a reasonable thing to try?
Secondly, does anyone have a suggestion of how to actually do the random noise addition outside of my region of interest?

Cheers,

Steinar


---

Steinar Halldorsson

Postdoctorate Fellow

Rosenthal lab

The Francis Crick Institute
London, United Kingdom

The Francis Crick Institute Limited is a registered charity in England and Wales no. 1140062 and a company registered in England and Wales no. 06885462, with its registered office at 1 Midland Road London NW1 1AT
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20181102/118a50a4/attachment-0001.html>