[3dem] Spiky class averages

[Sjors Scheres]

Hi Reza,
These are signs of weak signals. Better images will help, but if you're
stuck with these data, you could also limit the resolution of data used in
the E-step.
HTH,
Sjors

> Hi,
>
> We're getting spiky class averages with Relion 2.1. I'm assuming these are
> coming form over refinement(?). Other than playing with the regularization
> parameter, is there another way of controlling this?
>
>
> Best wishes,
> Reza
>
>
> Reza Khayat, PhD
> Assistant Professor
> City College of New York
> Department of Chemistry
> New York, NY 10031
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-- 
Sjors Scheres
MRC Laboratory of Molecular Biology
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