[3dem] Spiky class averages

[Gabriel Lander]

don't purify your proteins from porcupines?

> On Mar 16, 2018, at 8:50 AM, Reza Khayat <rkhayat at ccny.cuny.edu> wrote:
> 
> Hi,
> 
> We're getting spiky class averages with Relion 2.1. I'm assuming these are coming form over refinement(?). Other than playing with the regularization parameter, is there another way of controlling this?
> 
> Best wishes,
> Reza
> 
> Reza Khayat, PhD
> Assistant Professor 
> City College of New York
> Department of Chemistry
> New York, NY 10031
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