[3dem] Gatan 914 holder - should shutter close further than this?

Wim Hagen hagen at embl.de
Fri Aug 10 06:52:51 PDT 2018

Dear Teige,

Leopard print is typically caused by temperature problems, when the holder not being baked properly, or the clipping not making full contact.
Shutter problems would show contamination by large ice crystals picked up during holder insertion through air with the shutter not completely shielding.


Wim Hagen
EMBL Heidelberg

> On Aug 10, 2018, at 3:44 PM, Matthews-Palmer, Teige Rowan Seal <t.matthews-palmer14 at imperial.ac.uk> wrote:
> Dear 3DEMers,
> While troubleshooting some “leopard print” ice contamination on a Gatan 914 holder, we noticed that the underside of the cryoshield/shutter does not close 'all the way' (you can see the grid).
> I wanted to know from other users of 914 holders, does the underside of your shutter look like this when closed, or does it slide further to completely seal away the grid?
> An alternative cause of the contamination is a leak through the shutter rod assembly.
> Any thoughts welcome!
> Thanks,
> Teige
> <20180810_115423.jpeg>
> <20180810_115707.jpeg>
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