<html><head><meta http-equiv="content-type" content="text/html; charset=utf-8"></head><body dir="auto"><div></div><div>Dear Teige,</div><div><br></div><div>Leopard print is typically caused by temperature problems, when the holder not being baked properly, or the clipping not making full contact.</div><div>Shutter problems would show contamination by large ice crystals picked up during holder insertion through air with the shutter not completely shielding.</div><div><br></div><div>Best,</div><div><br></div><div>Wim Hagen</div><div>EMBL Heidelberg</div><div><br>On Aug 10, 2018, at 3:44 PM, Matthews-Palmer, Teige Rowan Seal <<a href="mailto:t.matthews-palmer14@imperial.ac.uk">t.matthews-palmer14@imperial.ac.uk</a>> wrote:<br><br></div><blockquote type="cite"><div>
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Dear 3DEMers,
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<div class="">While troubleshooting some “leopard print” ice contamination on a Gatan 914 holder, we noticed that the <b class="">underside</b> of the cryoshield/shutter does not close 'all the way' (you can see the grid).</div>
<div class="">I wanted to know from other users of 914 holders, does the underside of your shutter look like this when closed, or does it slide further to completely seal away the grid?</div>
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<div class="">An alternative cause of the contamination is a leak through the shutter rod assembly.</div>
<div class="">Any thoughts welcome!</div>
<div class="">Thanks,</div>
<div class="">Teige</div>
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<div class=""><20180810_115423.jpeg></div>
<div class=""><20180810_115707.jpeg></div>
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