[3dem] negative staining artifact

Sacha De Carlo sacha.decarlo at dectris.com
Sun Sep 24 14:40:42 PDT 2017


Looks like UA precipitate.

Best regards
Sacha

Sent from my mobile device
-----------------------------
Sacha De Carlo, Ph.D.
Business Development EM
Dectris.com

On 24 Sep 2017, at 23:26, Linda Sandblad <linda.sandblad at umu.se> wrote:

Hi all experts!!

I know, this do not look nice, but I would like to hear your opinion about
this artifact. Tiny dark spots covering both sample and background, do you
know what it is, were it comes from and how to avoid it? We have this bad
background time to time from different labs…. It is a “normal” negative
staining protocol: copper grids, formvar, carbon, glow discharge, UA.

I’m happy for your suggestions, Best Linda
<161111-100000X-0021.jpg>

Linda Sandblad
……………………………………………….
Department of Molecular Biology
Umeå University
+46 709324936

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