[3dem] negative staining artifact

[Gary Ren]

Hi Linda,
You may want to try this protocol to see if it could be helpful to you

https://www.jove.com/video/51087/optimized-negative-staining-high-throughput-protocol-for-examining

Best wishes
Gary 


Sent from my iPhone

> On Sep 24, 2017, at 2:25 PM, Linda Sandblad <linda.sandblad at umu.se> wrote:
> 
> Hi all experts!!
> 
> I know, this do not look nice, but I would like to hear your opinion about this artifact. Tiny dark spots covering both sample and background, do you know what it is, were it comes from and how to avoid it? We have this bad background time to time from different labs…. It is a “normal” negative staining protocol: copper grids, formvar, carbon, glow discharge, UA.
> 
> I’m happy for your suggestions, Best Linda
> 
> <161111-100000X-0021.jpg>
> 
> Linda Sandblad
> ……………………………………………….
> Department of Molecular Biology
> Umeå University
> +46 709324936
> 
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