[3dem] negative staining artifact

[Jaap Brink]

Thought the same. Maybe old UA or out in the light too long?

Cheers,

Jaap
----
Jacob Brink Ph.D.
TEM Product Manager Life Sciences
JEOL USA, Inc., Peabody MA 01960
(978) 317-9561

Sent from mobile device. Please excuse typos and this reply's brevity as I'm using all thumbs to type :-)

On Sep 24, 2017, at 3:41 PM, Sacha De Carlo <sacha.decarlo at dectris.com<mailto:sacha.decarlo at dectris.com>> wrote:

Looks like UA precipitate.

Best regards
Sacha

Sent from my mobile device
-----------------------------
Sacha De Carlo, Ph.D.
Business Development EM
Dectris.com<http://Dectris.com>

On 24 Sep 2017, at 23:26, Linda Sandblad <linda.sandblad at umu.se<mailto:linda.sandblad at umu.se>> wrote:

Hi all experts!!
I know, this do not look nice, but I would like to hear your opinion about this artifact. Tiny dark spots covering both sample and background, do you know what it is, were it comes from and how to avoid it? We have this bad background time to time from different labs.... It is a "normal" negative staining protocol: copper grids, formvar, carbon, glow discharge, UA.
I'm happy for your suggestions, Best Linda
<161111-100000X-0021.jpg>

Linda Sandblad
.......................................................
Department of Molecular Biology
Ume? University
+46 709324936

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