[3dem] Needing expert advice on cryosectioning for Tokuyasu method)
vlad_speransky at tedpella.com
Mon Oct 16 07:57:58 PDT 2017
Is there an image you could share? Your description suggests the methylcellulose possibly getting old, but could be other things.
Vlad Speransky, PhD
Life Sciences Product Specialist
Ted Pella, Inc.
530-243-2200 ext. 266
<mailto:vlad_speransky at tedpella.com> vlad_speransky at tedpella.com
From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of qiaohui.yang at ymail.com
Sent: Sunday, October 15, 2017 2:28 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Needing expert advice on cryosectioning for Tokuyasu method)
I have been working on trying to master the technique of cryosectioning and immunogoldlabelling. My embedded sections in (mixture of 1% methycellulose and 1.15UA) on final image look good but there seems to many round blobs like bubbles that persist on/under my sections. Would appreciate if experts in this technique would be able to give some advice on how to rectify this. Thanks!
Kind regards to all,
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