[3dem] Needing expert advice on cryosectioning for Tokuyasu method)

[Vlad Speransky]

Hi Qiaohui,

 

Is there an image you could share? Your description suggests the methylcellulose possibly getting old, but could be other things. 

 

Best wishes,

Vlad

 

Vlad Speransky, PhD

Life Sciences Product Specialist

Ted Pella, Inc.

 <http://www.tedpella.com/> http://www.tedpella.com/ 

530-243-2200 ext. 266

Cell 530-768-3953

 <mailto:vlad_speransky at tedpella.com> vlad_speransky at tedpella.com

 

 

 

 

From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of qiaohui.yang at ymail.com
Sent: Sunday, October 15, 2017 2:28 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Needing expert advice on cryosectioning for Tokuyasu method)

 

Hi everyone,

 

 

I have been working on trying to master the technique of cryosectioning and immunogoldlabelling. My embedded sections in (mixture of 1% methycellulose and 1.15UA) on final image look good but there seems to many round blobs like bubbles that persist on/under my sections. Would appreciate if experts in this technique would be able to give some advice on how to rectify this. Thanks!

 

Kind regards to all,

Qiaohui

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