[3dem] Needing expert advice on cryosectioning for Tokuyasu method)

[qiaohui.yang at ymail.com]

Hi everyone,

I have been working on trying to master the technique of cryosectioning and immunogoldlabelling. My embedded sections in (mixture of 1% methycellulose and 1.15UA) on final image look good but there seems to many round blobs like bubbles that persist on/under my sections. Would appreciate if experts in this technique would be able to give some advice on how to rectify this. Thanks!
Kind regards to all,Qiaohui

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