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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link=blue vlink=purple><div class=WordSection1><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Hi Qiaohui,<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Is there an image you could share? Your description suggests the methylcellulose possibly getting old, but could be other things. <o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Best wishes,<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Vlad<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Vlad Speransky, PhD<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Life Sciences Product Specialist<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Ted Pella, Inc.<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'><a href="http://www.tedpella.com/"><span style='color:#1F497D'>http://www.tedpella.com/</span></a> <o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>530-243-2200 ext. 266<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'>Cell 530-768-3953<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'><a href="mailto:vlad_speransky@tedpella.com"><span style='color:#1F497D'>vlad_speransky@tedpella.com</span></a><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><b><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>From:</span></b><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> 3dem [mailto:3dem-bounces@ncmir.ucsd.edu] <b>On Behalf Of </b>qiaohui.yang@ymail.com<br><b>Sent:</b> Sunday, October 15, 2017 2:28 AM<br><b>To:</b> 3dem@ncmir.ucsd.edu<br><b>Subject:</b> [3dem] Needing expert advice on cryosectioning for Tokuyasu method)<o:p></o:p></span></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Hi everyone,<o:p></o:p></p><div id="yMail_cursorElementTracker_1508059310024"><p class=MsoNormal><o:p> </o:p></p></div><div id="yMail_cursorElementTracker_1508059316098"><p class=MsoNormal><o:p> </o:p></p></div><div id="yMail_cursorElementTracker_1508059363391"><p class=MsoNormal>I have been working on trying to master the technique of cryosectioning and immunogoldlabelling. My embedded sections in (mixture of 1% methycellulose and 1.15UA) on final image look good but there seems to many round blobs like bubbles that persist on/under my sections. Would appreciate if experts in this technique would be able to give some advice on how to rectify this. Thanks!<o:p></o:p></p></div><div id="yMail_cursorElementTracker_1508059639311"><p class=MsoNormal><o:p> </o:p></p></div><div id="yMail_cursorElementTracker_1508059642652"><p class=MsoNormal>Kind regards to all,<o:p></o:p></p></div><div id="yMail_cursorElementTracker_1508059651320"><p class=MsoNormal style='margin-bottom:12.0pt'>Qiaohui<o:p></o:p></p><div id="ymail_android_signature"><p class=MsoNormal><a href="https://overview.mail.yahoo.com/mobile/?.src=Android">Sent from Yahoo Mail on Android</a><o:p></o:p></p></div></div></div></body></html>