[3dem] Overcoming orientation preference issue

[Jiang,Qiu-Xing]

Dear yang,
Besides other tricks suggested here, in our hands we noticed that our chemically oxidized carbon films have different surface property from the glow-discharged films and can change the orientational distribution by introducing a different interaction profile. A detailed procedure for chemical oxidation and cleaning is available in https://www.ncbi.nlm.nih.gov/pubmed/24457027 . It might provide a different alternative.
Best luck.
Qiu-Xing

-
From: 3dem <3dem-bounces at ncmir.ucsd.edu<mailto:3dem-bounces at ncmir.ucsd.edu>> on behalf of Yang Li <yanglixtal at gmail.com<mailto:yanglixtal at gmail.com>>
Date: Wednesday, May 17, 2017 at 8:46 AM
To: "3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>" <3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>>
Subject: [3dem] Overcoming orientation preference issue

Dear colleagues,

We have a protein sample that suffers from severe orientation preference, that most of the particles cluster into two distinct orientations. This way we have to collect large amounts of data in order to obtain enough effective particles, which hiders us from reaching high resolution. We have tried to make thicker ice or adding tiny amount of detergent such as Tween20, but not working very well so far. I wonder if there are any tricks we can try to overcome this orientation preference issue? Thank you in advance for suggestions!

Best,
Yang
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20170517/c5b61a41/attachment.html>