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<div>Dear yang, </div>
<div>Besides other tricks suggested here, in our hands we noticed that our chemically oxidized carbon films have different surface property from the glow-discharged films and can change the orientational distribution by introducing a different interaction profile.
A detailed procedure for chemical oxidation and cleaning is available in <a href="https://www.ncbi.nlm.nih.gov/pubmed/24457027">https://www.ncbi.nlm.nih.gov/pubmed/24457027</a> . It might provide a different alternative. </div>
<div>Best luck. </div>
<div>Qiu-Xing</div>
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<span style="font-weight:bold">From: </span>3dem <<a href="mailto:3dem-bounces@ncmir.ucsd.edu">3dem-bounces@ncmir.ucsd.edu</a>> on behalf of Yang Li <<a href="mailto:yanglixtal@gmail.com">yanglixtal@gmail.com</a>><br>
<span style="font-weight:bold">Date: </span>Wednesday, May 17, 2017 at 8:46 AM<br>
<span style="font-weight:bold">To: </span>"<a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a>><br>
<span style="font-weight:bold">Subject: </span>[3dem] Overcoming orientation preference issue<br>
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<div dir="ltr">Dear colleagues,
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<div>We have a protein sample that suffers from severe orientation preference, that most of the particles cluster into two distinct orientations. This way we have to collect large amounts of data in order to obtain enough effective particles, which hiders us
from reaching high resolution. We have tried to make thicker ice or adding tiny amount of detergent such as Tween20, but not working very well so far. I wonder if there are any tricks we can try to overcome this orientation preference issue? Thank you in advance
for suggestions!</div>
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<div>Best,</div>
<div>Yang </div>
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