[3dem] Overcoming orientation preference issue

[Yang Li]

Dear colleagues,

We have a protein sample that suffers from severe orientation preference,
that most of the particles cluster into two distinct orientations. This way
we have to collect large amounts of data in order to obtain enough
effective particles, which hiders us from reaching high resolution. We have
tried to make thicker ice or adding tiny amount of detergent such as
Tween20, but not working very well so far. I wonder if there are any tricks
we can try to overcome this orientation preference issue? Thank you in
advance for suggestions!

Best,
Yang
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