[3dem] Overcoming orientation preference issue

[Penczek, Pawel A]

You can try to collect data at a small tilt.  Thin ice of course. 

Regards,
Pawel

> On May 17, 2017, at 7:46 AM, Yang Li <yanglixtal at gmail.com> wrote:
> 
> Dear colleagues,
> 
> We have a protein sample that suffers from severe orientation preference, that most of the particles cluster into two distinct orientations. This way we have to collect large amounts of data in order to obtain enough effective particles, which hiders us from reaching high resolution. We have tried to make thicker ice or adding tiny amount of detergent such as Tween20, but not working very well so far. I wonder if there are any tricks we can try to overcome this orientation preference issue? Thank you in advance for suggestions!
> 
> Best,
> Yang 
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