[3dem] Liposomes in ice

Reza Khayat rkhayat at ccny.cuny.edu
Fri May 12 07:30:36 PDT 2017


Hi,


We are preparing extruded liposomes (200nm). Problem is we're getting very few liposomes in ice. The starting lipid concentration (DOPG: https://avantilipids.com/product/840475/) is 4mg/ml.


Reza


Reza Khayat, PhD
Assistant Professor
City College of New York
Department of Chemistry
New York, NY 10031
________________________________
From: Ludtke, Steven J <sludtke at bcm.edu>
Sent: Friday, May 12, 2017 10:27 AM
To: Reza Khayat
Subject: Re: [3dem] Liposomes in ice

You'll have to define exactly what you mean by liposomes. Extruded SUVs are one of our favorite test/training specimens because they are so easy to prepare and freeze. When most people say liposome, they mean something much larger. You'll need to give some details if you want useful advice. What are the lipid constituents, how large are the liposomes, how are they prepared, etc.   Would also be useful to see a representative image or two.

On May 12, 2017, at 9:18 AM, Reza Khayat <rkhayat at ccny.cuny.edu<mailto:rkhayat at ccny.cuny.edu>> wrote:

Hi,

We're trying to visualize liposomes in ice but are having some serious difficulty. Can anyone suggest some tricks that may be helpful? Thanks.

Best wishes,
Reza

Reza Khayat, PhD
Assistant Professor
City College of New York
Department of Chemistry
New York, NY 10031


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