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<p>Hi,</p>
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<p>We are preparing extruded liposomes (200nm). Problem is we're getting very few liposomes in ice. The starting lipid concentration (DOPG:
<a href="https://avantilipids.com/product/840475/">https://avantilipids.com/product/840475/</a>) is 4mg/ml.</p>
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<p>Reza<br>
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Reza Khayat, PhD
<div>Assistant Professor </div>
<div>City College of New York</div>
<div>Department of Chemistry</div>
<div>New York, NY 10031</div>
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<div id="divRplyFwdMsg" dir="ltr"><font style="font-size:11pt" color="#000000" face="Calibri, sans-serif"><b>From:</b> Ludtke, Steven J <sludtke@bcm.edu><br>
<b>Sent:</b> Friday, May 12, 2017 10:27 AM<br>
<b>To:</b> Reza Khayat<br>
<b>Subject:</b> Re: [3dem] Liposomes in ice</font>
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<div>You'll have to define exactly what you mean by liposomes. Extruded SUVs are one of our favorite test/training specimens because they are so easy to prepare and freeze. When most people say liposome, they mean something much larger. You'll need to give
some details if you want useful advice. What are the lipid constituents, how large are the liposomes, how are they prepared, etc. Would also be useful to see a representative image or two.
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<div class="">On May 12, 2017, at 9:18 AM, Reza Khayat <<a href="mailto:rkhayat@ccny.cuny.edu" class="">rkhayat@ccny.cuny.edu</a>> wrote:</div>
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Hi,</div>
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We're trying to visualize liposomes in ice but are having some serious difficulty. Can anyone suggest some tricks that may be helpful? Thanks.</div>
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Best wishes,</div>
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Reza<br class="">
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Reza Khayat, PhD
<div class="">Assistant Professor </div>
<div class="">City College of New York</div>
<div class="">Department of Chemistry</div>
<div class="">New York, NY 10031</div>
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Steven Ludtke, Ph.D.</div>
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Co-Director National Center For Macromolecular Imaging <span class="Apple-converted-space"> </span>(<a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__ncmi.bcm.edu&d=DgMFAg&c=4NmamNZG3KTnUCoC6InoLJ6KV1tbVKrkZXHRwtIMGmo&r=1DzJFW0v6TgEhkW1gy_-ke-RbtvS1fzEbD5_hcb9Up0&m=swGrDni_bnbj0SDRv5hphQJtdOkdoQ-0OVRvykOGmk0&s=vMVLCZ2x3qws1sikvDenN7wwVKbc5CZdCoKuHKU36L8&e=" class="">ncmi.bcm.edu</a>)</div>
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