[3dem] Liposomes in ice

[Parent, Kristin]

Hi Reza,

We've had great luck with this. What are the size of your liposomes? Ours have been smaller sizes less than 500nm.

I've had better luck with glow discharged grids than plasma cleaned ones for getting the lipids into the holes (longer discharge helps too) I also manually freeze instead of using a vitrobot. Quantifoil R2/2 work well. For some really large liposomes we had better success with 3.5/1 grids since they have bigger holes.

Other than that there wasn't anything special. Buffer was just 10 mM tris with 10mM magnesium.

Hope this helps. Kristin



Sent from my iPhone

On May 12, 2017, at 10:18 AM, Reza Khayat <rkhayat at ccny.cuny.edu<mailto:rkhayat at ccny.cuny.edu>> wrote:


Hi,


We're trying to visualize liposomes in ice but are having some serious difficulty. Can anyone suggest some tricks that may be helpful? Thanks.


Best wishes,

Reza


Reza Khayat, PhD
Assistant Professor
City College of New York
Department of Chemistry
New York, NY 10031


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