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<div>Hi Reza,</div>
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<div id="AppleMailSignature">We've had great luck with this. What are the size of your liposomes? Ours have been smaller sizes less than 500nm. </div>
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<div id="AppleMailSignature">I've had better luck with glow discharged grids than plasma cleaned ones for getting the lipids into the holes (longer discharge helps too) I also manually freeze instead of using a vitrobot. Quantifoil R2/2 work well. For some
really large liposomes we had better success with 3.5/1 grids since they have bigger holes. </div>
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<div id="AppleMailSignature">Other than that there wasn't anything special. Buffer was just 10 mM tris with 10mM magnesium.</div>
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<div id="AppleMailSignature">Hope this helps. Kristin </div>
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Sent from my iPhone</div>
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On May 12, 2017, at 10:18 AM, Reza Khayat <<a href="mailto:rkhayat@ccny.cuny.edu">rkhayat@ccny.cuny.edu</a>> wrote:<br>
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<p>Hi,</p>
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<p>We're trying to visualize liposomes in ice but are having some serious difficulty. Can anyone suggest some tricks that may be helpful? Thanks.</p>
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<p>Best wishes,</p>
<p>Reza<br>
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Reza Khayat, PhD
<div>Assistant Professor </div>
<div>City College of New York</div>
<div>Department of Chemistry</div>
<div>New York, NY 10031</div>
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