[3dem] instructional videos

Jensen, Grant J. jensen at caltech.edu
Wed Mar 29 19:18:19 PDT 2017

Dear colleagues:

In light of the recent NIH call for applications to develop cryo-EM educational materials (https://grants.nih.gov/grants/guide/rfa-files/RFA-RM-17-004.html), Matthijn Vos (Sr. Manager Customer Enablement and Application Specialist at Thermo Fischer) and I would like to let everyone know that we are already deep into the process of adding to my theoretical course “Getting started in cryo-EM” (http://cryo-em-course.caltech.edu/) a new set of practical videos that teach the workflow of single particle analysis from sample prep through microscope alignment and data collection. We will cover both the Krios and Arctica systems.

More specifically, we are working on videos covering (i) introduction to the Krios and Arctica instruments and user interface, (ii) daily microscope check, (iii) plunge-freezing using a Vitrobot, (iv) sample insertion and screening, (v) choosing data collection parameters and (vi) actual data collection using EPU with both Falcon and K2 direct electron detectors. Finally we will also be recording a common troubleshooting module. Like my theory videos, these practical videos will assume that the viewers are completely new to cryo-EM.

We are on course to start posting finished videos this fall, followed by formal training exercises and tests that students can do to develop and then demonstrate proficiency, step by step. This course will also form the basis for practical courses to come that will require completion of this online course as a prerequisite.

In 2018 we plan to add similar instruction for tomography, plus a facility manager and "train-the-trainer" series that will cover full microscope alignment, performance checks, routine maintenance, advanced troubleshooting, phase plates and possibly even cryo-FIB.  We hope cryo-EM students both new and old will find these videos helpful.

Please feel free to contact us with any questions or comments.

Wishing you the best,

Grant Jensen
Matthijn Vos (Matthijn.VosATfei.com)

Grant Jensen
Professor of Biology and Biophysics and Investigator, HHMI
California Institute of Technology, Mail code 114-96
1200 E. California Blvd.
Pasadena, CA  91125
626-395-8827 (phone)
626-395-5730 (fax)
office location: 359 Broad (northwest corner of campus)

Administrative Assistant Karin Mallard
626-395-8893 (phone)

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