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<span style="font-size: 10.5pt; font-family: -webkit-standard, serif;">Dear colleagues:</span></p>
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<span style="font-size: 10.5pt; font-family: -webkit-standard, serif;">In light of the recent NIH call for applications to develop cryo-EM educational materials (<a href="https://grants.nih.gov/grants/guide/rfa-files/RFA-RM-17-004.html" style="color: purple;">https://grants.nih.gov/grants/guide/rfa-files/RFA-RM-17-004.html</a>),
Matthijn Vos (Sr. Manager Customer Enablement and Application Specialist at Thermo Fischer) and I would like to let everyone know that we are already deep into the process of adding to my theoretical course “Getting started in cryo-EM” (<a href="http://cryo-em-course.caltech.edu/" style="color: purple;">http://cryo-em-course.caltech.edu/</a>)
a new set of practical videos that teach the workflow of single particle analysis from sample prep through microscope alignment and data collection. We will cover both the Krios and Arctica systems.<o:p></o:p></span></p>
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<span style="font-size: 10.5pt; font-family: -webkit-standard, serif;">More specifically, we are working on videos covering (i) introduction to the Krios and Arctica instruments and user interface, (ii) daily microscope check, (iii) plunge-freezing using a
Vitrobot, (iv) sample insertion and screening, (v) choosing data collection parameters and (vi) actual data collection using EPU with both Falcon and K2 direct electron detectors. Finally we will also be recording a common troubleshooting module. Like my theory
videos, these practical videos will assume that the viewers are completely new to cryo-EM.<o:p></o:p></span></p>
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<span style="font-size: 10.5pt; font-family: -webkit-standard, serif;">We are on course to start posting finished videos this fall, followed by formal training exercises and tests that students can do to develop and then demonstrate proficiency, step by step.
This course will also form the basis for practical courses to come that will require completion of this online course as a prerequisite.<o:p></o:p></span></p>
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<span style="font-size: 10.5pt; font-family: -webkit-standard, serif;">In 2018 we plan to add similar instruction for tomography, plus a facility manager and "train-the-trainer" series that will cover full microscope alignment, performance checks, routine maintenance,
advanced troubleshooting, phase plates and possibly even cryo-FIB. We hope cryo-EM students both new and old will find these videos helpful.<o:p></o:p></span></p>
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<span style="font-size: 10.5pt; font-family: -webkit-standard, serif;">Please feel free to contact us with any questions or comments. <o:p></o:p></span></p>
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<span style="font-size: 10.5pt; font-family: -webkit-standard, serif;">Wishing you the best,</span></p>
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<span style="font-size: 10.5pt; font-family: -webkit-standard, serif;">Grant Jensen <o:p></o:p></span></p>
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<span style="font-size: 10.5pt; font-family: -webkit-standard, serif;">Matthijn Vos (Matthijn.VosATfei.com)<o:p></o:p></span></p>
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<div>Grant Jensen</div>
<div>Professor of Biology and Biophysics and Investigator, HHMI</div>
<div>California Institute of Technology, Mail code 114-96</div>
<div>1200 E. California Blvd.</div>
<div>Pasadena, CA 91125</div>
<div>626-395-8827 (phone)</div>
<div>626-395-5730 (fax)</div>
<div>JensenATcaltech.edu</div>
<div>http://www.jensenlab.caltech.edu</div>
<div>office location: 359 Broad (northwest corner of campus)</div>
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<div>Administrative Assistant Karin Mallard </div>
<div>626-395-8893 (phone)</div>
<div>kmallardATcaltech.edu</div>
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