[3dem] Microscopy & Microanalysis Annual Meeting 2017
bullitt at bu.edu
Tue Jan 31 05:02:00 PST 2017
My most sincere apologies to all scientists whose travel is impacted by the absurd and harmful actions of the new US administration. Here is hoping that our strong efforts to change this policy are quickly successful.
I have attached here an announcement of the Microscopy & Microanalysis annual meeting, which this year includes celebration of the 75th anniversary of the Microscopy Society of America and the 50th anniversary of the Microanalysis Society.
I bring special attention to the educational and career-building aspects of the meeting for early career (student, post-doc) scientists, including awards that are available to support their attendance at the meeting. Travel and meeting registration awards are available, judged from submitted abstracts, and awards are given during the meeting, based on poster presentations. Please see www.microscopy.org/MandM/2017/meetings/apply_award.cfm
There is also a pre-meeting congress for early career professionals (see below)
Abstract deadline is Wednesday, February 15 (meeting: August 6-10)
http://www.microscopy.org/MandM/2017/ (Safari browser does not work well - try any other)
Here are some meeting highlights:
Anniversary Lectures by Pioneering Figures in Microscopy & Microanalysis
MSA 75th Anniversary Lecture in the Biological Sciences:
Development of High-resolution TEM for Imaging Native, Radiation-sensitive Biomolecules <http://www.microscopy.org/MandM/2017/program/Development_of_High_Resolution_paper.pdf>
Robert M. Glaeser, Lawrence Berkeley National Laboratory; University of California, Berkeley
MSA 75th Anniversary Lecture in the Physical Sciences:
Smarter Than an iPhone: The Emergence of the Modern Microscope <http://www.microscopy.org/MandM/2017/program/Smarter_than_an_iPhone_paper.pdf>
Ondrej L. Krivanek, Nion R&D; Arizona State University
Biological Sciences Symposia
B01 Gina Sosinsky Memorial Symposium: Imaging of Cellular Communications
Bernard Heymann, Esther Bullitt, Alasdair Steven
Gina Sosinskyï¿½s recent passing represents a severe loss. Gina was a valuable contributor to the 3DEM community and a delightful person. We are organizing this symposium to honor her and her work on cellular communication. One of her main interests was the junctions between eukaryotic cells, and how these serve both structural and communication functions. The former is essential to maintain the integrity of tissues, while the latter ensures propagation of signals between cells. Gina made important contributions to how gap junctions form selective channels between cells, and couple their cytoplasms to allow solute exchange. Adherens junctions and desmosomes provide mechanical contacts involved in transmitting force signals. Tight junctions offer selectivity to the passage of solutes in the spaces between cells. Retinoschisin is involved in maintaining the layered structure of the retina. Synaptic transmission affords the communication of information. The extracellular matrix forms a scaffolding for these and other proteins in the interstices between cells. Defects in these proteins cause debilitating diseases. The symposium will highlight the progress that has been made towards determining the structures and functions of proteins mediating cell-cell interactions using electron microscopy, light microscopy and other visualization techniques.
B03: Imaging the Biology of Cells and Tissues: Just Do It Right
Eduardo Rosa-Molinar, Jay Potts
This symposium will serve as a forum for the exchange of information, ideas, and knowledge regarding rigor and reproducibility in imaging sciences, a topic of increasing frequency and importance. Assuring confidence in and reproducibility of imaging results and their interpretation requires thoughtful consideration of the microscopic and experimental controls and procedures, detailed reporting of experimental design, methodologies, and means of analysis, as well as a review of a long and rich literature in the imaging sciences. Using case studies, panelists will discuss the most significant and recurring imaging issues, strive to reach a consensus about the extent to which rigor and reproducibility is a problem, and, suggest individual and organizational means of addressing the concern. Target attendees include undergraduate and graduate students, post-doctoral researchers, and scientists of all levels of experience/expertise and related backgrounds.
B04 3D and Intravital Imaging in Development and Beyond
David Entenberg, Kevin Eliceri, Sandra Rugonyi
While conventional optical imaging tools like 2D in vitro assays offer the ability to tightly and reproducibly control experimental conditions, they do not adequately reflect the topography or diversity of environments encountered by cells in vivo. 3D and intravital imaging can remove these restrictions and restore the heterogeneity of environments present in the living organism. For example, 3D organoid cultures free cells from the culture dishï¿½s two dimensional confines revealing more physiologically relevant motility and extracellular matrix interactions; whole mount imaging preserves the structural integrity and spatial arrangement of the living organism; and intravital imaging further restores multiple host cell interactions, connection to lymphatic and vascular circuits and regulatory signals from distant organs. These increasingly more physiological environs come at the price of greatly increased challenges for the design of experiments and extraction of interpretable information from them. This session is intended to be a forum for highlighting new developments and techniques in the art and science of 3D and intravital imaging as well as what can be learned from their application to areas ranging from development to pathology.
B05 Pharmaceuticals and Medical Science
Bridget Carragher, Jason Mantei
This symposium will present diverse content related to the manufacturing and use of pharmaceuticals and medical products. There will be detailed case studies that demonstrate the use of advanced techniques to address the unique problems that arise during drug discovery, vaccine research, formulation, biocompatibility, production, product life cycle, and eventual patient use. There will also be in-depth technical presentations covering the development of methods specially optimized for use with these real-world material and biological systems. The research may involve use of hybrid or correlative techniques that are inclusive and generally include any of the instruments and methods found in the exposition. As always, contributed papers for both platform and poster presentation are encouraged.
B06 3D Structures of Macromolecular Assemblies, Cellular Organelles, and Whole Cells
Deborah F. Kelly, Elizabeth Wright, Teresa Ruiz
Our understanding of the 3D structure and functional subtleties of cells, microorganisms and macromolecular assemblies has skyrocketed due to recent advances in EM imaging technology and hybrid methodologies. This symposium will highlight structural studies of cells, microorganisms and macromolecules using state-of-the-art high resolution techniques. These techniques include, electron tomography; electron crystallography; single particle cryo-EM; helical reconstruction; STEM; AFM; X-ray crystallography, and molecular modeling. Biological topics of interest include: cellular architectures, metabolism, trafficking, and division; gene regulation, transcription, and translation; host-pathogen interactions and virus structure; In situ studies using TEM and SEM; and all aspects of structure-function studies of biological assemblies.
B07 Bridging the Gap: Technologies and Methods for Correlative Light and Charged Particle Microscopy of Biological Systems
James A.J. Fitzpatrick, Matthew S. Joens, Joshua Z. Rappoport
Correlating light and charged particle (both electron, ion and x-ray) microscopy methodologies serves to bridge the multi-scale gap that hinders both the two- and three-dimensional analyses of rare cellular and sub-cellular level events that remain beyond reach due to the diffraction limit of light. The need to correlate information obtained from both types of datasets has proven a significant challenge, but has evolved in recent years with the advent of new probes, processing techniques, and detectors with substantially increased sensitivity. We seek to highlight the scientific innovations that address the correlation of light and charged particle microscopy of biological samples and soft materials. This symposium is intended to be a forum for the dissemination of correlative workflows and advanced sample preparation methods. Invited papers would include those describing novel approaches for correlating information from multiple light modalities with x-ray, electron and ion microscopy datasets. Of particular interest are innovative developments of new CLEM probes and contrast agents, advanced methods for ultrastructural preservation and dynamic in situ measurements. Target attendees will include engineers and scientists from all levels of bio-imaging expertise and all related backgrounds.
B08 Utilizing Microscopy for Research and Diagnosis of Diseases in Humans, Plants and Animals
Gang (Greg) Ning, Ru-ching Hsia, Trace Christensen, Jon Charlesworth
Microscopy is not only useful but also critically important in the ongoing research, detection, diagnosis and treatment of disease. Advances that improve rapid and accurate detection and treatment often involve the use of various microscopic techniques. These varied techniques provide us with an improved ability to diagnose and research the origins, development and response of diseases in human, plant and animal specimens. This is an opportunity to share information on the investigation of pathogenic cells, tissues and entire organisms in clinical, diagnostic and research laboratories. Emphasis will be placed on using latest microscopy in both clinical and research laboratories.
2017 Pre-Meeting Congresses
Separate registration fee required; see registration information on website, and form for details (form available March 1, 2017).
See individual listings below for information on meals and breaks.
X60 Inaugural Pre-Meeting Congress for Early Career Professionals in Microscopy & Microanalysis
Saturday, August 5, 2017 * 8:30 AM to 5:00 PM
Included in Registration Fee: Breakfast, AM Break, Lunch, PM Break
(Offsite social gatherings are being planned for Friday and Saturday evenings, as well.)
Organized by MSA Student Council
Program Chair: William J. Bowman, Arizona State University
Program Co-Chair: A. Cameron Varano, Virginia Tech (Biological Sciences)
Program Co-chair: Janet L. Gbur, Case Western Reserve University (Physical Sciences)
Activities & Social Chair: Ethan L. Lawrence, Arizona State University
To commemorate MSA’s 75th anniversary, the Student Council is organizing an inaugural pre-meeting congress for early career professionals in microscopy & microanalysis, to be held on the Saturday preceding the M&M 2017 in St. Louis, MO. The event will be preceded by an informal get-together on Friday night, where participants can meet in a relaxed setting, and is scheduled to allow those interested to attend a Sunday short course or FIG-sponsored pre-meeting congress, or to enjoy a day in St. Louis with new friends.
This congress is organized by early career professionals, primarily for early career professionals, though all M&M registrants are welcome to attend. The pre-meeting will offer a highly interactive forum for participants to share cutting edge research, network, and engage with peers ahead of the main meeting. Invited speakers will be selected from among awardees to be honored at M&M 2017, giving attendees an opportunity to experience a sampling of the best research to be presented by their peers across scientific disciplines in biological science, physical science, analytical science and instrumentation. Contributed talks and posters will give attendees an opportunity to discuss their work with peers in an intimate and highly interactive setting. Further professional development opportunities will include a luncheon featuring a panel of recent graduates currently working in industry, academia, policy, and government labs.
X61 Focused Ion Beam Applications and Equipment Developments
Sunday, August 6, 2017 * 10:00 AM – 5:00 PM
Included in Registration Fee: Lunch, PM Break
Organized by the Focused Ion Beam (FIB) Focused Interest Group
Nicholas Antoniou, Revera
Srinivas Subramaniam, Intel Corporation
Focused Ion Beam technology is used in a variety of fields from electronics to life sciences. The applications space can be divided into categories such as cryogenic FIB, Direct-write lithography, 3D structure creation etc. The topics will be grouped together into sessions as follows:
TEM Sample prep
FIB Lithography and general patterning
Gas Assisted etching and deposition
One hour will be allotted to each category with 2-3 papers and 20 minutes of open discussion. At the end of the congress, posters will be set up for informal interaction with the authors and participants.
X62 Smaller, Faster, Better: New Instrumentation for Electron Microscopy
Sunday, August 6, 2017 * 8:30 AM – 5:00 PM
Included in Registration Fee: Breakfast, AM Break, Lunch, PM Break
Organized by the Aberration-Corrected Electron Microscopy (ACEM) Focused Interest Group
Paul Voyles, University of Wisconsin
Huolin Xin, Brookhaven National Laboratory
Juan-Carlo Idrobo, Oak Ridge National Laboratory
Phil Rice, IBM
Developments in instrumentation drive new science. Now, two decades after the first working aberration correctors, this pre-meeting congress will address the question, what’s next? Topic covered will include advances in: detectors, especially high-speed pixelated and segmented detectors; monochromators to achieve energy resolution below 10 meV; aberration correctors, especially at low voltage; fast beam manipulation, especially for compressed sensing; and high brightness sources. Example applications and supporting advances in data processing and simulation will also be included. The meeting will consist of invited talks and a poster session.
X63 Understanding Radiation Beam-Damage during Cryo-, ETEM, Gas- and Liquid-Cell Electron Microscopy
Sunday, August 6, 2017 * 8:30 AM – 5:00 PM
Included in Registration Fee: Breakfast, AM Break, Lunch, PM Break
Katherine Jungjohann, Sandia National Laboratories
Taylor Woehl, University of Maryland
Patricia Abellan, SuperSTEM Laboratory (UK)
Electron-beam induced radiation damage to gases, liquids, and vitrified ice is a technical problem for the electron microscopy (EM) study of natural and engineered structures as they exist in their ambient environments. This one-day congress is designed to provide insight into the processes that occur when a high-energy electron beam interacts with a material, its gas/liquid/solid embedding medium and the interfaces between them. Steady-state radiation conditions will be evaluated in terms of temperature variations, external probing, mass transport, and imaging modes for describing the reactive system. Speakers will highlight the electron-dose threshold tolerances, low-LET ionizing radiation effects, damage mechanisms, prevention, and control of radiation effects for small volume environmental studies. The format will consist of invited presentations from radiation chemists and individuals with significant contributions to understanding electron-beam effects for environmental EM and in-situ x-rays cells. The congress will feature a lunch-time poster session and a panel discussion to identify the major challenges going forward, and should be of interested to EM researchers in the areas of cryo-EM, ETEM, gas-cell EM and liquid-cell EM.
M&M 2017 Sunday Short Courses
Organizer: Elizabeth Wright, Emory University
These full-day courses run from 8:30 AM to 5:00 PM on Sunday, August 6, 2017.
A certificate of participation will be issued to each requesting participant, following the conclusion of the M&M 2017 meeting.
Two (2) Continuing Microscopy Education Units are available (registration fee $10 for members).
Morning and afternoon coffee breaks are included (breakfast and lunch are on your own).
Separate registration with additional fees is required (see registration form for more information). Registration will open on March 1, 2017.
X10 Specimen Preparation for Biological EM of Resin-embedded Samples: Cryo-methods, Correlative LM-EM and 3-D Imaging
Lead Instructor: Kent McDonald
In this course, we will review why cryo-techniques for biological specimen preparation are superior to conventional methods. We will discuss high-pressure freezing, freeze substitution, and preserving fluorescence in polymerized resiins. Persons taking this course should leave with a better understanding of biological EM cryotechniques and their role in different applications such as correlative LM - EM, EM tomography, EM immunolabeling, and as the best method for preservation of cellular fine structure. Specimen preparation procedures for resin-based 3-D imaging methods will also be discussed.
X-11 Immunolabeling Technology for Light and Electron Microscopy
Lead instructor: Caroline Miller
The requirements for successful immunohistochemical and immunocytochemical labeling vary widely with different biological systems. The optimal techniques for light-microscope labeling often differ greatly from those needed for electron microscopy. The basics of immunolabeling at the light- and electron-microscope levels will be presented, illustrated with examples from several different biological systems. Some of the more complex methods and applications used in electron microscopy will be discussed in depth. The course will cover specimen preparation, immunogold labeling and enhancement methods, multiple labeling and correlative LM/EM techniques.
X12 Practical Considerations for Image Analysis, ImageJ and Clemex Vision
Instructor: James Grande
This workshop covers a wide range of practical topics in the field of image analysis. Subjects will be covered in an easy-to-understand format so that users with little or no experience can understand how image analysis can provide extensive quantitative measurements that may lead to better understanding of material performance. Topics range from input devices to image-processing algorithms and how best to extract quantitative data. Treating image analysis as a problem-solving tool along with discerning key metrics within a microstructure is discussed through several real-life examples. Comparisons using ImageJ/Fiji and a commercial image analysis product will be demonstrated.
X-12 3-D Reconstruction with SerialEM and IMOD
Lead Instructor: Cindi Schwartz
This workshop will cover the use of SerialEM for data acquisition and IMOD for 3-D reconstruction and analysis. For SerialEM, topics include basic operation, low dose mode, STEM imaging, use of direct detector cameras, tilt series acquisition, montaging, and automatic acquisition from multiple areas. For IMOD, topics include reconstruction from single and dual-axis tilt series, alignment of slices or tomograms from serial sections, automated processing of multiple tilt series, and modeling and visualization. Cryo and room-temperature applications will be covered. Although the emphasis will be on biological samples, many points are applicable to materials science.
X14 Detectors: If You Can't Detect It, Then You Can't Measure It
Lead Instructor: Nestor J. Zaluzec
Imaging and spectroscopy have long played pivotal roles in characterization of materials in both the biological and physical sciences. Of course, a signal must absolutely be detectable from an object in order to make any observations or measurements. For this course, we will assemble a cadre of researchers from both academia and industry, who will discuss the principles of various imaging and spectroscopic detectors, their limitations, and future prospects and technologies. Topics will include optical sensors and single-particle sensors for photons, x-rays, electrons and ions, as well as electromagnetic-field sensors, and the use of these sensors as imaging/spectroscopic detectors.
X 15 Variable Pressure and Environmental Scanning Electron Microscopy: What Can They Do For Me?
Lead Instructor: John Mansfield
Variable-pressure scanning EM (VPSEM) and environmental scanning EM (ESEM), while readily available, are not used as frequently as they should be. This course will compare the structure, operation, and special detectors of the two. The practicality of x-ray spectroscopy will be examined. The unique contrast mechanisms in these microscopes will be discussed. The use of the VPSEM as an in-situ platform for hot, cold and mechanical testing experiments will also be covered. Applications experts from instrument manufacturers will be invited to describe new developments. A summary presentation comparing the VPSEM with conventional SEM will conclude the course.
Biological Sciences Tutorials
Organizer: Tommi A. White, University of Missouri
X42 CryoEM with Phase Plates
Radostin Danev, Max Planck Institute of Biochemistry (Germany)
Introduction to phase plates
Setting up cryo-tomography and single particle data acquisition with a phase plate
Processing of phase plate single particle data
X43 Practical Strategies for Cryo-CLEM Experiments
Cheri Hampton, Emory University
Available methodologies for Correlative Light and Electron Microscopy (CLEM)
Emphasis on cryo-fLM paired with cryo-EM/ET
Practical considerations and troubleshooting for biological sample preparation
Applications for virus-host interactions and virus structure including bacterial and mammalian systems
X44 Freeze Fracture, Deep-Etch & 3D Anaglyphs
Robyn Roth, Washington University
Esther Bullitt, Ph.D.
Dept. of Physiology & Biophysics
Boston University School of Medicine
700 Albany Street, Room W302
Boston, MA 02118-2526
Email: bullitt at bu.edu
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