[3dem] 3dem Digest, Vol 109, Issue 19

[Qiang Guo]

Hi Bill,

What's the size of the tilt serials after binning? As I remembered, for
SIRT, the image size must be a even value to perform, for WBP, it doesn't
matter.

Hopefully it helps.

Best
Qiang

2016-09-08 1:53 GMT+02:00 <3dem-request at ncmir.ucsd.edu>:

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>    1. SIRT (Bill & Sue Tivol)
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> Message: 1
> Date: Wed, 7 Sep 2016 16:51:50 -0700
> From: Bill & Sue Tivol <wtivol at sbcglobal.net>
> To: 3dem at ncmir.ucsd.edu
> Subject: [3dem] SIRT
> Message-ID: <DEDAA739-349C-42BD-823C-23EA63EE901D at sbcglobal.net>
> Content-Type: text/plain; charset="utf-8"
>
> Hi All,
>         I have a data set that I?d like to reconstruct with SIRT.  I
> started etomo and successfully got through the final alignment step,
> resulting in a well-aligned tilt series.  When I set up the Generate
> Tomogram step, clicking on SIRT, I chose iterations 20 and 30 to save.  The
> reconstruction failed, generating the error message shown below.  I also
> tried a WBP reconstruction on the same data set, using the aligned tilt
> series from the SIRT reconstruction, and it gave a reasonable result.  I do
> not know how to interpret the error message.  Could someone please let me
> know what I might do to get a successful SIRT reconstruction?  BTW, I also
> tried to use the command line tomo3d -S -a filename.tlt -i filename.ali -o
> filename.srec with appropriate parameters for thickness in z and iterations
> to save?a process I had used successfully on another computer?but I got a
> command-not-found error.  Is there a way to install part of etomo to get
> access to that command?  TIA for all help.
>                                                 Yours,
>                                                 Bill
>
>
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-- 
Qiang (John) Guo
Department of Molecular Structural Biology
Max Planck Institute of Biochemistry
Am Klopferspitz 18
82152 Martinsried
Germany
Email: guoq.tiger at gmail.com
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