<div dir="ltr"><div>Hi Bill,<br><br></div><div>What's the size of the tilt serials after binning? As I remembered, for SIRT, the image size must be a even value to perform, for WBP, it doesn't matter. <br><br></div><div>Hopefully it helps.<br><br></div><div>Best<br></div><div>Qiang<br></div></div><div class="gmail_extra"><br><div class="gmail_quote">2016-09-08 1:53 GMT+02:00 <span dir="ltr"><<a href="mailto:3dem-request@ncmir.ucsd.edu" target="_blank">3dem-request@ncmir.ucsd.edu</a>></span>:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">Send 3dem mailing list submissions to<br>
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1. SIRT (Bill & Sue Tivol)<br>
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Message: 1<br>
Date: Wed, 7 Sep 2016 16:51:50 -0700<br>
From: Bill & Sue Tivol <<a href="mailto:wtivol@sbcglobal.net">wtivol@sbcglobal.net</a>><br>
To: <a href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br>
Subject: [3dem] SIRT<br>
Message-ID: <<a href="mailto:DEDAA739-349C-42BD-823C-23EA63EE901D@sbcglobal.net">DEDAA739-349C-42BD-823C-<wbr>23EA63EE901D@sbcglobal.net</a>><br>
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Hi All,<br>
I have a data set that I?d like to reconstruct with SIRT. I started etomo and successfully got through the final alignment step, resulting in a well-aligned tilt series. When I set up the Generate Tomogram step, clicking on SIRT, I chose iterations 20 and 30 to save. The reconstruction failed, generating the error message shown below. I also tried a WBP reconstruction on the same data set, using the aligned tilt series from the SIRT reconstruction, and it gave a reasonable result. I do not know how to interpret the error message. Could someone please let me know what I might do to get a successful SIRT reconstruction? BTW, I also tried to use the command line tomo3d -S -a filename.tlt -i filename.ali -o filename.srec with appropriate parameters for thickness in z and iterations to save?a process I had used successfully on another computer?but I got a command-not-found error. Is there a way to install part of etomo to get access to that command? TIA for all help.<br>
Yours,<br>
Bill<br>
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</blockquote></div><br><br clear="all"><br>-- <br><div class="gmail_signature" data-smartmail="gmail_signature"><div dir="ltr">Qiang (John) Guo<br>Department of Molecular Structural Biology<br>Max Planck Institute of Biochemistry<br>Am Klopferspitz 18<br>82152 Martinsried<br>Germany<br>Email: <a href="mailto:guoq.tiger@gmail.com" target="_blank">guoq.tiger@gmail.com</a> <br></div></div>
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