[3dem] Shutter problems with Digital Micrograph ver 3.1

[Kaustuv Basu, Dr]

Keeping an eye on TEM Blank shutter monitor, go in GMS-Configure Camera. Check the Primary and Alternate Shutter Combination.Check if the alternate shutter is closed.Also, check the Insertion Delay,Retraction delay time.


For Orius, the shutter combination (pre-specimen).Check the insertion Delay, Retraction Delay and Auto retraction delay.


see if this work.


Kaustuv Basu,

Staff Scientist
Facility for Electron Microscopy Research
McGill University
Strathcona Anatomy & Dentistry Building
3640,Rue University ,Montreal,QC H3A 0C7
http://www.mcgill.ca/femr/contact
Tel: 514-398-6405 (Work)
        438-933-0614 (Cell)


________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Mike Strauss <mikestrauss13 at crystal.harvard.edu>
Sent: November 26, 2016 2:24 PM
To: rubinst1
Cc: 3dem at ncmir.ucsd.edu
Subject: Re: [3dem] Shutter problems with Digital Micrograph ver 3.1

What does the Blanker Monitor tell you?

On Thu, Nov 24, 2016 at 7:25 PM, rubinst1 <john.rubinstein at utoronto.ca<mailto:john.rubinstein at utoronto.ca>> wrote:
Thanks, Jaap.

We seem to have the same problem with both the Orius and the K2 (on the Orius it manifests as not being able to acquire a dark reference, although at one point we did a test and saw that we could radiation damage a stain specimen with the blanker ‘on’). The Orius has a separate control box so the coincidence would have to be two controllers malfunctioning at the same time as a software upgrade.

Best regards,
John



--
John Rubinstein
Molecular Structure and Function Program
The Hospital for Sick Children Research Institute
686 Bay Street, Rm. 20-9705
Toronto, ON
Canada
M5G 0A4
Tel: (+001) 416-813-7255<tel:416-813-7255>
Fax: (+001) 416-813-5022<tel:416-813-5022>
www.sickkids.ca/research/rubinstein<http://www.sickkids.ca/research/rubinstein>

On Nov 24, 2016, at 1:15 PM, Jaap Brink <jbrink at jeol.com<mailto:jbrink at jeol.com>> wrote:

John,

I have encountered several installs with DM version 3.1 and all seemed to behave as expected wrt blanking. Could it be a coincidental breakdown of the opto-coupler between the controller and the scope. Berkeley just had theirs go out on them ....

Happy Turkey day.

Jaap
----
Jacob Brink Ph.D.
TEM Product Manager Life Sciences
JEOL USA, Inc., Peabody MA 01960
(978) 317-9561<tel:%28978%29%20317-9561>

Sent from mobile device. Please excuse typos and this reply's brevity as I'm using all thumbs to type :-)

On Nov 24, 2016, at 12:05 PM, rubinst1 <john.rubinstein at utoronto.ca<mailto:john.rubinstein at utoronto.ca>> wrote:

Dear Colleagues,

We recently had Digital Micrograph on our Tecnai F20 (with a Gatan K2 Summit DDD and Orius CCD) upgraded from ver 2.3 to ver 3.1.
Since the upgrade we have had intermittent problems with the cameras not applying the microscope shutter/beam blanker when they should. When the shutter is not applied properly cryo-EM specimens are pre-exposed before images are acquired.

We are not exactly sure of the sequence of events that leads to the problem. However, once we notice there is a problem during a microscope session we can confirm that the camera isn’t applying the shutter properly because  (1) the voltage from the shutter cable is 0V (when it should be 5V to apply the shutter) and (2) the microscope gun deflector value correspond to no shutter being applied. Samir Benlekbir has worked out a sequence of steps (involving raising and lowering the microscope screen and starting and stopping the camera) that will correct the problem once it has been detected, but of course this shouldn’t be necessary.

Has anyone else had a similar problem caused by upgrading to Digital Micrograph 3.1? If you have, please let me know how you resolved it.

Thanks and best wishes,
John


--
John Rubinstein
Molecular Structure and Function Program
The Hospital for Sick Children Research Institute
686 Bay Street, Rm. 20-9705
Toronto, ON
Canada
M5G 0A4
Tel: (+001) 416-813-7255<tel:416-813-7255>
Fax: (+001) 416-813-5022<tel:416-813-5022>
www.sickkids.ca/research/rubinstein<http://www.sickkids.ca/research/rubinstein>

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