[3dem] Shutter problems with Digital Micrograph ver 3.1

[Mike Strauss]

What does the Blanker Monitor tell you?

On Thu, Nov 24, 2016 at 7:25 PM, rubinst1 <john.rubinstein at utoronto.ca>
wrote:

> Thanks, Jaap.
>
> We seem to have the same problem with both the Orius and the K2 (on the
> Orius it manifests as not being able to acquire a dark reference, although
> at one point we did a test and saw that we could radiation damage a stain
> specimen with the blanker ‘on’). The Orius has a separate control box so
> the coincidence would have to be two controllers malfunctioning at the same
> time as a software upgrade.
>
> Best regards,
> John
>
>
>
> --
> John Rubinstein
> Molecular Structure and Function Program
> The Hospital for Sick Children Research Institute
> 686 Bay Street, Rm. 20-9705
> Toronto, ON
> Canada
> M5G 0A4
> Tel: (+001) 416-813-7255
> Fax: (+001) 416-813-5022
> www.sickkids.ca/research/rubinstein
>
> On Nov 24, 2016, at 1:15 PM, Jaap Brink <jbrink at jeol.com> wrote:
>
> John,
>
> I have encountered several installs with DM version 3.1 and all seemed to
> behave as expected wrt blanking. Could it be a coincidental breakdown of
> the opto-coupler between the controller and the scope. Berkeley just had
> theirs go out on them ....
>
> Happy Turkey day.
>
> Jaap
> ----
> Jacob Brink Ph.D.
> TEM Product Manager Life Sciences
> JEOL USA, Inc., Peabody MA 01960
> (978) 317-9561
>
> Sent from mobile device. Please excuse typos and this reply's brevity as
> I'm using all thumbs to type :-)
>
> On Nov 24, 2016, at 12:05 PM, rubinst1 <john.rubinstein at utoronto.ca>
> wrote:
>
> Dear Colleagues,
>
> We recently had Digital Micrograph on our Tecnai F20 (with a Gatan K2
> Summit DDD and Orius CCD) upgraded from ver 2.3 to ver 3.1.
> Since the upgrade we have had intermittent problems with the cameras not
> applying the microscope shutter/beam blanker when they should. When the
> shutter is not applied properly cryo-EM specimens are pre-exposed before
> images are acquired.
>
> We are not exactly sure of the sequence of events that leads to the
> problem. However, once we notice there is a problem during a microscope
> session we can confirm that the camera isn’t applying the shutter properly
> because  (1) the voltage from the shutter cable is 0V (when it should be 5V
> to apply the shutter) and (2) the microscope gun deflector value correspond
> to no shutter being applied. Samir Benlekbir has worked out a sequence of
> steps (involving raising and lowering the microscope screen and starting
> and stopping the camera) that will correct the problem once it has been
> detected, but of course this shouldn’t be necessary.
>
> Has anyone else had a similar problem caused by upgrading to Digital
> Micrograph 3.1? If you have, please let me know how you resolved it.
>
> Thanks and best wishes,
> John
>
>
> --
> John Rubinstein
> Molecular Structure and Function Program
> The Hospital for Sick Children Research Institute
> 686 Bay Street, Rm. 20-9705
> Toronto, ON
> Canada
> M5G 0A4
> Tel: (+001) 416-813-7255
> Fax: (+001) 416-813-5022
> www.sickkids.ca/research/rubinstein
>
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