[3dem] Shutter problems with Digital Micrograph ver 3.1
hideki.shigematsu at riken.jp
Sun Nov 27 18:05:56 PST 2016
We have had a different issue with shutter function on GMS3.1 with our Tecnai Arctica. On our Arctica, the shutter function is working OK at least the camera had been inserted. Just because of there is no signal for screen position on our Arctica, once the camera had been retracted, shutter would not work with any of screen position. Our gatan service engineer said this is correct behavior, but I am asking local gatan to communicate with Chris Booth for this issue.
Hideki Shigematsu Ph. D.
RIKEN Center for Life Science Technologies
1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045 Japan
> 2016/11/25 午前3:25、rubinst1 <john.rubinstein at utoronto.ca> のメール：
> Thanks, Jaap.
> We seem to have the same problem with both the Orius and the K2 (on the Orius it manifests as not being able to acquire a dark reference, although at one point we did a test and saw that we could radiation damage a stain specimen with the blanker ‘on’). The Orius has a separate control box so the coincidence would have to be two controllers malfunctioning at the same time as a software upgrade.
> Best regards,
> John Rubinstein
> Molecular Structure and Function Program
> The Hospital for Sick Children Research Institute
> 686 Bay Street, Rm. 20-9705
> Toronto, ON
> M5G 0A4
> Tel: (+001) 416-813-7255
> Fax: (+001) 416-813-5022
>> On Nov 24, 2016, at 1:15 PM, Jaap Brink <jbrink at jeol.com> wrote:
>> I have encountered several installs with DM version 3.1 and all seemed to behave as expected wrt blanking. Could it be a coincidental breakdown of the opto-coupler between the controller and the scope. Berkeley just had theirs go out on them ....
>> Happy Turkey day.
>> Jacob Brink Ph.D.
>> TEM Product Manager Life Sciences
>> JEOL USA, Inc., Peabody MA 01960
>> (978) 317-9561
>> Sent from mobile device. Please excuse typos and this reply's brevity as I'm using all thumbs to type :-)
>> On Nov 24, 2016, at 12:05 PM, rubinst1 <john.rubinstein at utoronto.ca> wrote:
>>> Dear Colleagues,
>>> We recently had Digital Micrograph on our Tecnai F20 (with a Gatan K2 Summit DDD and Orius CCD) upgraded from ver 2.3 to ver 3.1.
>>> Since the upgrade we have had intermittent problems with the cameras not applying the microscope shutter/beam blanker when they should. When the shutter is not applied properly cryo-EM specimens are pre-exposed before images are acquired.
>>> We are not exactly sure of the sequence of events that leads to the problem. However, once we notice there is a problem during a microscope session we can confirm that the camera isn’t applying the shutter properly because (1) the voltage from the shutter cable is 0V (when it should be 5V to apply the shutter) and (2) the microscope gun deflector value correspond to no shutter being applied. Samir Benlekbir has worked out a sequence of steps (involving raising and lowering the microscope screen and starting and stopping the camera) that will correct the problem once it has been detected, but of course this shouldn’t be necessary.
>>> Has anyone else had a similar problem caused by upgrading to Digital Micrograph 3.1? If you have, please let me know how you resolved it.
>>> Thanks and best wishes,
>>> John Rubinstein
>>> Molecular Structure and Function Program
>>> The Hospital for Sick Children Research Institute
>>> 686 Bay Street, Rm. 20-9705
>>> Toronto, ON
>>> M5G 0A4
>>> Tel: (+001) 416-813-7255
>>> Fax: (+001) 416-813-5022
>>> 3dem mailing list
>>> 3dem at ncmir.ucsd.edu
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