[3dem] De novo model building from medium-resolution cryo EM maps

[Grigory Sharov]

Dear all,

I'm looking for a software tool for *de novo* model building (no homologous
structures available) based on a medium resolution density maps (5-8 A).
The idea is to get at least a C-alpha trace and map it onto known protein
sequence.

So far I tried Gorgon and e2pathwalker from EMAN2 without much luck and now
looking into Rosetta (not sure it is suitable for my resolution). I wonder
what other people are using for such task.


Best regards,
Grigory

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Grigory Sharov, Ph.D.
Institute of Genetics and Molecular and Cellular Biology
Integrated Structural Biology Department (CBI)
1, rue Laurent Fries
67404 Illkirch, France
tel. 03 69 48 51 00
e-mail: sharov at igbmc.fr
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