[3dem] 3dem Digest, Vol 101, Issue 16

[Cristina Paulino]

Dear Daniel,

Have you tried other grid metals? 
From my experience the sample drop spreads much better on its own to the other side when using gold grids rather then copper grids. 
The most extrem case is if you use a gold-coated holey carbon film. Here the drop (in most cases) distributes evenly on both sides of the grid after application on one side.  

Best,
Cristina



> On Jan 20, 2016, at 5:31 PM, 3dem-request at ncmir.ucsd.edu wrote:
> 
> Send 3dem mailing list submissions to
> 	3dem at ncmir.ucsd.edu <mailto:3dem at ncmir.ucsd.edu>
> 
> To subscribe or unsubscribe via the World Wide Web, visit
> 	https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem <https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem>
> or, via email, send a message with subject or body 'help' to
> 	3dem-request at ncmir.ucsd.edu <mailto:3dem-request at ncmir.ucsd.edu>
> 
> You can reach the person managing the list at
> 	3dem-owner at ncmir.ucsd.edu <mailto:3dem-owner at ncmir.ucsd.edu>
> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of 3dem digest..."
> Today's Topics:
> 
>   1. Re: blotting quantifoil from copper side (Mohamed Chami)
> 
> From: Mohamed Chami <mohamed.chami at unibas.ch <mailto:mohamed.chami at unibas.ch>>
> Subject: Re: [3dem] blotting quantifoil from copper side
> Date: January 20, 2016 at 5:31:19 PM GMT+1
> To: Levy Daniel <Daniel.Levy at curie.fr <mailto:Daniel.Levy at curie.fr>>, "3dem at ncmir.ucsd.edu <mailto:3dem at ncmir.ucsd.edu>" <3dem at ncmir.ucsd.edu <mailto:3dem at ncmir.ucsd.edu>>
> 
> 
> Dear Daniel,
> We had also this problem with Quantifoil grids because the paper filter doesn't have contact with the sample when you blot from the back side. The carbon film of Quantifoil's grids is thicker than the carbon film of Tedpella's grids. 2 tricks you can try:
> - Glow discharges the both sides of the grids
> or
> - Put the sample (3 or 4 ul) of the sample on the carbon side and put also a little droplet of sample on the other side (blotting side) just by touching the middle of the grid with the tip of the pipette in order to make a bridge between the sample and the blotting side. 
> Hope it will help
> Best
> Mohamed
> 
> Mohamed Chami, PhD
> Bio-EM lab
> Center for Cellular Imaging and NanoAnalytics (C-CINA)
> Biozentrum, University of Basel
> Mattenstrasse 26, 
> CH-4058 Basel
> Switzerland
> Tel.: +41-61-387 32 24
> 
> Fax: +41-61-387 39 86
> E-mail: mohamed.chami at unibas.ch <mailto:mohamed.chami at unibas.ch>
> http://www.c-cina.unibas.ch <http://www.c-cina.unibas.ch/>
> 
> 
> From: 3dem [3dem-bounces at ncmir.ucsd.edu <mailto:3dem-bounces at ncmir.ucsd.edu>] on behalf of Levy Daniel [Daniel.Levy at curie.fr <mailto:Daniel.Levy at curie.fr>]
> Sent: Wednesday, January 20, 2016 4:29 PM
> To: 3dem at ncmir.ucsd.edu <mailto:3dem at ncmir.ucsd.edu>
> Subject: [3dem] blotting quantifoil from copper side
> 
> Dear all
> We have some difficulties to get homogenous ice when we blot our quantifoil grids with a Leica EM GP. We have low concentrated samples and we want to blot from the side opposite to the drop (the copper side). Our conditions worked well with holey formvar Ted Pella grids but not with quantifoil. We tried different grades of paper (1 or 4)  blotting conditions and glow discharged conditions. Any advice will be helpful.
>  
> Best
> daniel
>  
> Daniel Lévy
> UMR CNRS 168 Institut Curie
> 11 rue P.M.Curie
> F-75231 Paris Cedex 05
> France
> Phone 33 1 56 24 67 82
> Fax 33 1 40 51 06 36
> http://umr168.curie.fr/en/Levy-group <http://umr168.curie.fr/en/Levy-group>
>  
> 
> 
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu <mailto:3dem at ncmir.ucsd.edu>
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem <https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem>
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20160120/d4a64335/attachment-0001.html>