<html><head><meta http-equiv="Content-Type" content="text/html charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Dear Daniel,<div class=""><br class=""></div><div class="">Have you tried other grid metals? </div><div class="">From my experience the sample drop spreads much better on its own to the other side when using gold grids rather then copper grids. </div><div class="">The most extrem case is if you use a gold-coated holey carbon film. Here the drop (in most cases) distributes evenly on both sides of the grid after application on one side. </div><div class=""><br class=""></div><div class="">Best,</div><div class="">Cristina</div><div class=""><br class=""></div><div class=""><br class=""></div><div class=""><br class=""><div><blockquote type="cite" class=""><div class="">On Jan 20, 2016, at 5:31 PM, <a href="mailto:3dem-request@ncmir.ucsd.edu" class="">3dem-request@ncmir.ucsd.edu</a> wrote:</div><br class="Apple-interchange-newline"><div class=""><span style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">Send 3dem mailing list submissions to</span><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><span class="Apple-tab-span" style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: pre; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;"> </span><a href="mailto:3dem@ncmir.ucsd.edu" style="color: purple; text-decoration: underline; font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">3dem@ncmir.ucsd.edu</a><br style="font-family: HelveticaNeue; 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font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><span style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">or, via email, send a message with subject or body 'help' to</span><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><span class="Apple-tab-span" style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: pre; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;"> </span><a href="mailto:3dem-request@ncmir.ucsd.edu" style="color: purple; text-decoration: underline; font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">3dem-request@ncmir.ucsd.edu</a><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><span style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">You can reach the person managing the list at</span><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><span class="Apple-tab-span" style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: pre; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;"> </span><a href="mailto:3dem-owner@ncmir.ucsd.edu" style="color: purple; text-decoration: underline; font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">3dem-owner@ncmir.ucsd.edu</a><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><span style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">When replying, please edit your Subject line so it is more specific</span><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><span style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">than "Re: Contents of 3dem digest..."</span><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><span style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">Today's Topics:</span><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><span style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class=""> 1. Re: blotting quantifoil from copper side (Mohamed Chami)</span><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><div style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; margin: 0px;" class=""><span style="font-family: -webkit-system-font, 'Helvetica Neue', Helvetica, sans-serif; color: rgb(127, 127, 127);" class=""><b class="">From:<span class="Apple-converted-space"> </span></b></span><span style="font-family: -webkit-system-font, 'Helvetica Neue', Helvetica, sans-serif;" class="">Mohamed Chami <<a href="mailto:mohamed.chami@unibas.ch" style="color: purple; text-decoration: underline;" class="">mohamed.chami@unibas.ch</a>><br class=""></span></div><div style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; margin: 0px;" class=""><span style="font-family: -webkit-system-font, 'Helvetica Neue', Helvetica, sans-serif; color: rgb(127, 127, 127);" class=""><b class="">Subject:<span class="Apple-converted-space"> </span></b></span><span style="font-family: -webkit-system-font, 'Helvetica Neue', Helvetica, sans-serif;" class=""><b class="">Re: [3dem] blotting quantifoil from copper side</b><br class=""></span></div><div style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; margin: 0px;" class=""><span style="font-family: -webkit-system-font, 'Helvetica Neue', Helvetica, sans-serif; color: rgb(127, 127, 127);" class=""><b class="">Date:<span class="Apple-converted-space"> </span></b></span><span style="font-family: -webkit-system-font, 'Helvetica Neue', Helvetica, sans-serif;" class="">January 20, 2016 at 5:31:19 PM GMT+1<br class=""></span></div><div style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; margin: 0px;" class=""><span style="font-family: -webkit-system-font, 'Helvetica Neue', Helvetica, sans-serif; color: rgb(127, 127, 127);" class=""><b class="">To:<span class="Apple-converted-space"> </span></b></span><span style="font-family: -webkit-system-font, 'Helvetica Neue', Helvetica, sans-serif;" class="">Levy Daniel <<a href="mailto:Daniel.Levy@curie.fr" style="color: purple; text-decoration: underline;" class="">Daniel.Levy@curie.fr</a>>, "<a href="mailto:3dem@ncmir.ucsd.edu" style="color: purple; text-decoration: underline;" class="">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu" style="color: purple; text-decoration: underline;" class="">3dem@ncmir.ucsd.edu</a>><br class=""></span></div><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><div style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; direction: ltr; font-family: Tahoma; font-size: 10pt;" class="">Dear Daniel,<div class="">We had also this problem with Quantifoil grids because the paper filter doesn't have contact with the sample when you blot from the back side. The carbon film of Quantifoil's grids is thicker than the carbon film of Tedpella's grids. 2 tricks you can try:</div><div class="">- Glow discharges the both sides of the grids</div><div class="">or</div><div class="">- Put the sample (3 or 4 ul) of the sample on the carbon side and put also a little droplet of sample on the other side (blotting side) just by touching the middle of the grid with the tip of the pipette in order to make a bridge between the sample and the blotting side. </div><div class="">Hope it will help</div><div class="">Best</div><div class="">Mohamed</div><div class=""><br class=""></div><div class=""><p class="MsoNormal" style="margin: 0cm 0cm 3.5pt; font-size: 11pt; font-family: Calibri, sans-serif;"><span style="font-size: 14pt; font-family: Arial; color: rgb(141, 179, 226);" class="">Mohamed Chami, PhD<o:p class=""></o:p></span></p><p class="MsoNormal" style="margin: 0cm 0cm 3.5pt; font-size: 11pt; font-family: Calibri, sans-serif;"><span style="font-family: Arial; color: rgb(141, 179, 226);" class="">Bio-EM lab<o:p class=""></o:p></span></p><p class="MsoNormal" style="margin: 0cm 0cm 3.9pt; font-size: 11pt; font-family: Calibri, sans-serif;"><span style="font-family: Arial; color: rgb(141, 179, 226);" class="">Center for Cellular Imaging and NanoAnalytics (C-CINA)<o:p class=""></o:p></span></p><p class="MsoNormal" style="margin: 0cm 0cm 3.9pt; font-size: 11pt; font-family: Calibri, sans-serif;"><span style="font-size: 13pt; font-family: Arial; color: rgb(141, 179, 226);" class="">Biozentrum, University of Basel</span><span style="font-size: 16pt; font-family: Arial; color: rgb(141, 179, 226);" class=""><o:p class=""></o:p></span></p><p class="MsoNormal" style="margin: 0cm 0cm 3.3pt; font-size: 11pt; font-family: Calibri, sans-serif;"><span style="font-family: Arial; color: rgb(141, 179, 226);" class="">Mattenstrasse 26,
CH-4058 Basel<o:p class=""></o:p></span></p><p class="MsoNormal" style="margin: 0cm 0cm 3.3pt; font-size: 11pt; font-family: Calibri, sans-serif;"><span style="font-family: Arial; color: rgb(141, 179, 226);" class="">Switzerland<o:p class=""></o:p></span></p><p class="MsoNormal" style="margin: 0cm 0cm 3.3pt; font-size: 11pt; font-family: Calibri, sans-serif;"><span style="font-family: Arial; color: rgb(141, 179, 226);" class="">Tel.: +41-61-387 32 24
<o:p class=""></o:p></span></p><p class="MsoNormal" style="margin: 0cm 0cm 3.3pt; font-size: 11pt; font-family: Calibri, sans-serif;"><span style="font-family: Arial; color: rgb(141, 179, 226);" class="">Fax: +41-61-387 39 86<o:p class=""></o:p></span></p><p class="MsoNormal" style="margin: 0cm 0cm 3.3pt; font-size: 11pt; font-family: Calibri, sans-serif;"><span style="font-family: Arial; color: rgb(141, 179, 226);" class="">E-mail: </span><a href="mailto:mohamed.chami@unibas.ch" style="color: purple; text-decoration: underline;" class=""><span style="font-family: Arial; color: rgb(217, 149, 148); text-decoration: none;" class="">mohamed.chami@unibas.ch</span></a><span style="font-family: Arial; color: rgb(217, 149, 148);" class=""><o:p class=""></o:p></span></p><p class="MsoNormal" style="margin: 0cm 0cm 3.3pt; font-size: 11pt; font-family: Calibri, sans-serif;"><span style="font-family: Arial; color: rgb(217, 149, 148);" class=""><a href="http://www.c-cina.unibas.ch/" style="color: purple; text-decoration: underline;" class="">http://www.c-cina.unibas.ch</a><o:p class=""></o:p></span></p></div><div class=""><br class=""></div><div class=""><br class=""><div style="font-family: 'Times New Roman'; font-size: 16px;" class=""><hr tabindex="-1" class=""><div id="divRpF869098" style="direction: ltr;" class=""><font face="Tahoma" size="2" class=""><b class="">From:</b><span class="Apple-converted-space"> </span>3dem [<a href="mailto:3dem-bounces@ncmir.ucsd.edu" style="color: purple; text-decoration: underline;" class="">3dem-bounces@ncmir.ucsd.edu</a>] on behalf of Levy Daniel [<a href="mailto:Daniel.Levy@curie.fr" style="color: purple; text-decoration: underline;" class="">Daniel.Levy@curie.fr</a>]<br class=""><b class="">Sent:</b><span class="Apple-converted-space"> </span>Wednesday, January 20, 2016 4:29 PM<br class=""><b class="">To:</b><span class="Apple-converted-space"> </span><a href="mailto:3dem@ncmir.ucsd.edu" style="color: purple; text-decoration: underline;" class="">3dem@ncmir.ucsd.edu</a><br class=""><b class="">Subject:</b><span class="Apple-converted-space"> </span>[3dem] blotting quantifoil from copper side<br class=""></font><br class=""></div><div class=""></div><div class=""><div class="WordSection1"><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">Dear all</div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span lang="EN-US" class="">We have some difficulties to get homogenous ice when we blot our quantifoil grids with a Leica EM GP. We have low concentrated samples and we want to blot from the side opposite to the drop (the copper side). Our conditions worked well with holey formvar Ted Pella grids but not with quantifoil. We tried different grades of paper (1 or 4) blotting conditions and glow discharged conditions. Any advice will be helpful.</span></div><p class="MsoNormal" style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;"><span lang="EN-US" class=""> </span></p><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span lang="EN-US" class="">Best</span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span lang="EN-US" class="">daniel</span></div><p class="MsoNormal" style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;"><span lang="EN-US" class=""> </span></p><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span class="">Daniel Lévy</span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span class="">UMR CNRS 168 Institut Curie</span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span class="">11 rue P.M.Curie</span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span class="">F-75231 Paris Cedex 05</span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span class="">France</span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span class="">Phone 33 1 56 24 67 82</span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span class="">Fax 33 1 40 51 06 36</span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span class=""><a href="http://umr168.curie.fr/en/Levy-group" style="color: purple; text-decoration: underline;" class="">http://umr168.curie.fr/en/Levy-group</a></span></div><p class="MsoNormal" style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;"> </p></div></div></div></div></div><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><span style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">_______________________________________________</span><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><span style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">3dem mailing list</span><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><a href="mailto:3dem@ncmir.ucsd.edu" style="color: purple; text-decoration: underline; font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">3dem@ncmir.ucsd.edu</a><br style="font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" style="color: purple; text-decoration: underline; font-family: HelveticaNeue; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class="">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a></div></blockquote></div><br class=""></div></body></html>