[3dem] molecular weight issue for CryoEM

[Ben Engel]

Hi Prakash,

If you have access to a microscope with one installed, try using a Volta phase plate.

Best,
Ben



> On Apr 23, 2016, at 9:34 PM, prakash shukla <life.prakash at gmail.com> wrote:
> 
> Dear All,
> 
> I have purify a complex of three proteins.They are interacting with each other but the molecular weight of protein 150 kDa. I want to do cryoEM for complex as for literature molecular weight of protein should be more than 300 kDa. how should i proceed for Cryo EM. Suggestions are most welcome.
> 
> Regards
> 
> Prakash
> 
>    
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