[3dem] molecular weight issue for CryoEM

Reza Khayat rkhayat at ccny.cuny.edu
Sat Apr 23 13:39:16 PDT 2016


Hi,


I50kDa is possible, but very challenging. Start in stain and see if ice is a possibility.


Reza


Reza Khayat, PhD
Assistant Professor
City College of New York
Department of Chemistry
New York, NY 10031
________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of prakash shukla <life.prakash at gmail.com>
Sent: Saturday, April 23, 2016 3:34 PM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] molecular weight issue for CryoEM

Dear All,

I have purify a complex of three proteins.They are interacting with each other but the molecular weight of protein 150 kDa. I want to do cryoEM for complex as for literature molecular weight of protein should be more than 300 kDa. how should i proceed for Cryo EM. Suggestions are most welcome.

Regards

Prakash


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