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<p>Hi,<br>
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<p>I50kDa is possible, but very challenging. Start in stain and see if ice is a possibility. <br>
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<p>Reza<br>
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Reza Khayat, PhD
<div>Assistant Professor </div>
<div>City College of New York</div>
<div>Department of Chemistry</div>
<div>New York, NY 10031</div>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" color="#000000" style="font-size:11pt"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of prakash shukla <life.prakash@gmail.com><br>
<b>Sent:</b> Saturday, April 23, 2016 3:34 PM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] molecular weight issue for CryoEM</font>
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<div dir="ltr">Dear All,
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<div>I have purify a complex of three proteins.They are interacting with each other but the molecular weight of protein 150 kDa. I want to do cryoEM for complex as for literature molecular weight of protein should be more than 300 kDa. how should i proceed
for Cryo EM. Suggestions are most welcome.</div>
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<div>Regards</div>
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<div>Prakash</div>
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