[3dem] ruthenium tetroxide

[David Morgan]

Hi,

I would store the aliquots much colder, at -80 or even in liquid 
nitrogen.  We had a student here who tried storage of OsO4 at 
temperatures closer to -20 and those aliquots seemed to slowly oxidize 
while being stored.  She switched to keeping them in a liquid nitrogen 
storage dewar and didn't have that problem.

--
            David Gene Morgan
         Electron Microscopy Center
             047D Simon Hall
             IU Bloomington
          812 856 1457 (office)
          812 856 3221 (3200)
     http://www.bio.indiana.edu/~cryo/


Quoting "Gibson, Kimberley" <kimberley.gibson at yale.edu>:

> Hi Frank,
>
> I would assume that you could treat RuO4 similar to OsO4, in which
> case, assume that it will react rather quickly once exposed to air.
> If the solution turns dark, then it should be discarded.
>
> Upon opening a vial, you can make smaller aliquots of the 0.5%
> solution in eppendorf tubes and store them immediately at -20 C until
> needed. Thaw only those aliquots that you would need.
>
> If you store the liquid solution in a fridge, it should be placed in
> a plastic, sealable container. It might be better to store the liquid
> in -20 aliquots as it will likely 'go off' overnight if stored at
> only 4C once the ampule has been opened.
>
> Hopefully this helps. I admittedly, have not worked with RuO4, but
> chemically it sounds similar enough to Osmium tetroxide.
>
> Best,
>
> Kimberley Gibson
>
> Post-Graduate Associate, Charles Sindelar Lab
>
> Dept. Molecular Biophysics and Biochemistry
> Yale University
> SHMC-E15
> 333 Cedar St.
> New Haven, CT
> 06520-8024
> USA
>
> Lab (203) 737-4723
> Email: kimberley.gibson at yale.edu
> ________________________________
> From: 3dem [3dem-bounces at ncmir.ucsd.edu] on behalf of Frank Polzer
> [frank.polzer at physik.hu-berlin.de]
> Sent: May 28, 2015 13:08
> To: 3dem at ncmir.ucsd.edu
> Subject: [3dem] ruthenium tetroxide
>
> Dear all,
>
> I recently purchased RuO4 in aqueous solution (0.5%) from EMS which
> comes in glass ampules.
>
> Can anyone tell me how long after opening the ampules the RuO4 can be
> used if being stored in dark and refrigerated?
>
> Also, do you have any suggestion what sort of secondary container one
> could use to store the opened glass ampules in the fridge?
>
>
> Thank you very much for your help in advance.
>
> Cheers,
>
> Frank
>
>
>