[3dem] ruthenium tetroxide

[Johannes Haataja]

Hi all,
 We usually transfer the RuO4 solution to glass vials after breaking the
ampulle and store them inside larger vial (matroska style) in fridge. I
would not use eppendorf tubes, since RuO4 might permeate through plastic
materials*. Even the teflon caps that are used with glass vials tend to
slowly corrode due to exposure to RuO4 atmosphere. 

 - J.

* "Solutions will turn black with exposure to organic material or strong
U.V. light"
http://www.polysciences.com/skin/frontend/default/polysciences/pdf/TDS%
20320.pdf


to, 2015-05-28 kello 14:16 -0400, David Morgan kirjoitti:
> Hi,
> 
> I would store the aliquots much colder, at -80 or even in liquid 
> nitrogen.  We had a student here who tried storage of OsO4 at 
> temperatures closer to -20 and those aliquots seemed to slowly oxidize 
> while being stored.  She switched to keeping them in a liquid nitrogen 
> storage dewar and didn't have that problem.
> 
> --
>             David Gene Morgan
>          Electron Microscopy Center
>              047D Simon Hall
>              IU Bloomington
>           812 856 1457 (office)
>           812 856 3221 (3200)
>      http://www.bio.indiana.edu/~cryo/
> 
> 
> Quoting "Gibson, Kimberley" <kimberley.gibson at yale.edu>:
> 
> > Hi Frank,
> >
> > I would assume that you could treat RuO4 similar to OsO4, in which
> > case, assume that it will react rather quickly once exposed to air.
> > If the solution turns dark, then it should be discarded.
> >
> > Upon opening a vial, you can make smaller aliquots of the 0.5%
> > solution in eppendorf tubes and store them immediately at -20 C until
> > needed. Thaw only those aliquots that you would need.
> >
> > If you store the liquid solution in a fridge, it should be placed in
> > a plastic, sealable container. It might be better to store the liquid
> > in -20 aliquots as it will likely 'go off' overnight if stored at
> > only 4C once the ampule has been opened.
> >
> > Hopefully this helps. I admittedly, have not worked with RuO4, but
> > chemically it sounds similar enough to Osmium tetroxide.
> >
> > Best,
> >
> > Kimberley Gibson
> >
> > Post-Graduate Associate, Charles Sindelar Lab
> >
> > Dept. Molecular Biophysics and Biochemistry
> > Yale University
> > SHMC-E15
> > 333 Cedar St.
> > New Haven, CT
> > 06520-8024
> > USA
> >
> > Lab (203) 737-4723
> > Email: kimberley.gibson at yale.edu
> > ________________________________
> > From: 3dem [3dem-bounces at ncmir.ucsd.edu] on behalf of Frank Polzer
> > [frank.polzer at physik.hu-berlin.de]
> > Sent: May 28, 2015 13:08
> > To: 3dem at ncmir.ucsd.edu
> > Subject: [3dem] ruthenium tetroxide
> >
> > Dear all,
> >
> > I recently purchased RuO4 in aqueous solution (0.5%) from EMS which
> > comes in glass ampules.
> >
> > Can anyone tell me how long after opening the ampules the RuO4 can be
> > used if being stored in dark and refrigerated?
> >
> > Also, do you have any suggestion what sort of secondary container one
> > could use to store the opened glass ampules in the fridge?
> >
> >
> > Thank you very much for your help in advance.
> >
> > Cheers,
> >
> > Frank
> >
> >
> >
> 
> 
> 
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