[3dem] Manual data collection with Falcon camera

[Grassucci, Robert]

Dear Chuang,
May I suggest Leginon but use the queuing option for selecting your exposure targets?  As I am told it works with a falcon camera and be more efficient than manual data collection.  We do this routinely with our K2 camera when we have problematic samples that do not want to spread evenly.  I also know of many groups using SerialEM but I do not have as much experience with that.  Perhaps there are other groups that can comment?  Good luck.
Regards,
Bob


From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of Liu, Chuang
Sent: Wednesday, June 03, 2015 9:31 AM
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Manual data collection with Falcon camera



 Dear all


Our microscope is equipped with camera Falcon and automatic data collection software EPU. As everybody knows, data collection for single particle is much easier than before with such settings.  But for our filament project, EPU haven't give us such overwhelming effect as in single particle field.  In our situation,the filament's concentration is kept low to avoid overlapping,so the chance to shoot a good tube is not high when automatically collecting data hole by hole.  We still prefer manual data collection by taking an image at low mag(4300x) and moving stage to the target area for higher mag imaging.And here it seems that this method is more efficient.

The problem now is that we can't use Falcon's movie mode while not running EPU.  For us, it seems that movie mode is only controlled by the software EPU. So does anybody have experiences on such situation that either collecting data manually with Falcon's movie mode or collecting data while running EPU but under supervision?

Thanks a lot.
Regards

Chuang


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