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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Dear Chuang,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">May I suggest Leginon but use the queuing option for selecting your exposure targets? As I am told it works with a falcon camera and be more efficient than
manual data collection. We do this routinely with our K2 camera when we have problematic samples that do not want to spread evenly. I also know of many groups using SerialEM but I do not have as much experience with that. Perhaps there are other groups
that can comment? Good luck.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Regards,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Bob<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> 3dem [mailto:3dem-bounces@ncmir.ucsd.edu]
<b>On Behalf Of </b>Liu, Chuang<br>
<b>Sent:</b> Wednesday, June 03, 2015 9:31 AM<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> [3dem] Manual data collection with Falcon camera<o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Calibri","sans-serif";color:black"> </span><span style="font-size:14.0pt;font-family:"Calibri","sans-serif";color:#222222">Dear all</span><span style="font-family:"Calibri","sans-serif";color:black"><o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Calibri","sans-serif";color:#222222"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Calibri","sans-serif";color:#222222"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-size:14.0pt;font-family:"Calibri","sans-serif";color:#222222">Our microscope is equipped with camera Falcon and automatic data collection software EPU. As everybody knows, data collection for single
particle is much easier than before with such settings. But for our filament project, EPU haven't give us such overwhelming effect as in single particle field. In our situation,the filament's concentration is kept low to avoid overlapping,so the chance to
shoot a good tube is not high when automatically collecting data hole by hole. We still prefer manual data collection by taking an image at low mag(4300x) and moving stage to the target area for higher mag imaging.And here it seems that this method is more
efficient.</span><span style="font-family:"Calibri","sans-serif";color:#222222"><o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Calibri","sans-serif";color:#222222"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-size:14.0pt;font-family:"Calibri","sans-serif";color:#222222">The problem now is that we can't use Falcon's movie mode while not running EPU. For us, it seems that movie mode is only controlled
by the software EPU. So does anybody have experiences on such situation that either collecting data manually with Falcon's movie mode or collecting data while running EPU but under supervision?</span><span style="font-family:"Calibri","sans-serif";color:#222222"><o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Calibri","sans-serif";color:#222222"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-size:14.0pt;font-family:"Calibri","sans-serif";color:#222222">Thanks a lot.</span><span style="font-family:"Calibri","sans-serif";color:#222222"><o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-size:14.0pt;font-family:"Calibri","sans-serif";color:#222222">Regards</span><span style="font-family:"Calibri","sans-serif";color:#222222"><o:p></o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-family:"Calibri","sans-serif";color:#222222"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="background:white"><span style="font-size:14.0pt;font-family:"Calibri","sans-serif";color:#222222">Chuang</span><span style="font-family:"Arial","sans-serif";color:#222222"> <o:p></o:p></span></p>
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<p style="background:white"><span style="font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
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